2cdn
From Proteopedia
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| - | [[Image:2cdn.gif|left|200px]]<br /><applet load="2cdn" size=" | + | [[Image:2cdn.gif|left|200px]]<br /><applet load="2cdn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2cdn, resolution 1.90Å" /> | caption="2cdn, resolution 1.90Å" /> | ||
'''CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ADENYLATE KINASE COMPLEXED WITH TWO MOLECULES OF ADP AND MG'''<br /> | '''CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ADENYLATE KINASE COMPLEXED WITH TWO MOLECULES OF ADP AND MG'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2CDN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] Known structural/functional Site: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2CDN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] Known structural/functional Site: <scene name='pdbsite=AC1:Adp+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CDN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:34:11 2008'' |
Revision as of 08:34, 3 February 2008
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CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS ADENYLATE KINASE COMPLEXED WITH TWO MOLECULES OF ADP AND MG
Overview
The crystal structure of Mycobacterium tuberculosis adenylate kinase, (MtAK) in complex with two ADP molecules and Mg2+ has been determined at, 1.9 A resolution. Comparison with the solution structure of the enzyme, obtained in the absence of substrates, shows significant conformational, changes of the LID and NMP-binding domains upon substrate binding. The, ternary complex represents the state of the enzyme at the start of the, backward reaction (ATP synthesis). The structure is consistent with a, direct nucleophilic attack of a terminal oxygen from the acceptor ADP, molecule on the beta-phosphate from the donor substrate, and both the, geometry and the distribution of positive charge in the active site, support the hypothesis of an associative mechanism for phosphoryl, transfer.
About this Structure
2CDN is a Single protein structure of sequence from Mycobacterium tuberculosis with and as ligands. Active as Adenylate kinase, with EC number 2.7.4.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of Mycobacterium tuberculosis adenylate kinase in complex with two molecules of ADP and Mg2+ supports an associative mechanism for phosphoryl transfer., Bellinzoni M, Haouz A, Grana M, Munier-Lehmann H, Shepard W, Alzari PM, Protein Sci. 2006 Jun;15(6):1489-93. Epub 2006 May 2. PMID:16672241
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