2chm

From Proteopedia

Revision as of 08:35, 3 February 2008

CRYSTAL STRUCTURE OF N2 SUBSTITUTED PYRAZOLO PYRIMIDINONES-A FLIPPED BINDING MODE IN PDE5

Overview

Sildenafil, (5-[2-ethoxy-5-(4-methyl-1-piperazinylsulfonyl)phenyl]-1-methyl-3-n-propyl, -1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidin-7-one), a potent and selective, phosphodiesterase type 5 (PDE5) inhibitor, provided the first oral, treatment for male erectile dysfunction. The objective of the work, reported in this paper was to combine high levels of PDE5 potency and, selectivity with high and dose-independent oral bioavailability, to, minimize the impact on the C(max) of any interactions with coadministered, drugs in the clinic. This goal was achieved through identification of a, lower clearance series with a high absorption profile, by replacing the, 5'-piperazine sulfonamide in the sildenafil template with a 5'-methyl, ketone. This novel series provided compounds with low metabolism in human, hepatocytes, excellent caco-2 flux, and the potential for good aqueous, solubility. The in vivo oral and iv pharmacokinetic profiles of example, compounds confirmed the high oral bioavailability predicted from these in, vitro screens., 5-(5-Acetyl-2-butoxy-3-pyridinyl)-3-ethyl-2-(1-ethyl-3-azetidinyl)-2,6-dih, ydro-7H-pyrazolo[4,3-d]pyrimidin-7-one (2) was selected for progression, into the clinic.

About this Structure

2CHM is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

A novel series of potent and selective PDE5 inhibitors with potential for high and dose-independent oral bioavailability., Allerton CM, Barber CG, Beaumont KC, Brown DG, Cole SM, Ellis D, Lane CA, Maw GN, Mount NM, Rawson DJ, Robinson CM, Street SD, Summerhill NW, J Med Chem. 2006 Jun 15;49(12):3581-94. PMID:16759100

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