2iu4
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2iu4.gif|left|200px]]<br /><applet load="2iu4" size=" | + | [[Image:2iu4.gif|left|200px]]<br /><applet load="2iu4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2iu4, resolution 1.96Å" /> | caption="2iu4, resolution 1.96Å" /> | ||
'''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''<br /> | '''DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2IU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with SO4 and HIQ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2IU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HIQ:'>HIQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:38:14 2008'' |
Revision as of 08:38, 3 February 2008
|
DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ
Overview
Dihydroxyacetone (Dha) kinases are a novel family of kinases with, signaling and metabolic functions. Here we report the x-ray structures of, the transcriptional activator DhaS and the coactivator DhaQ and, characterize their function. DhaQ is a paralog of the Dha binding Dha, kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional, activator. DhaQ and DhaS form a stable complex that in the presence of Dha, activates transcription of the Lactococcus lactis dha operon. Dha, covalently binds to DhaQ through a hemiaminal bond with a histidine and, thereby induces a conformational change, which is propagated to the, surface via a cantilever-like structure. DhaS binding protects an inverted, repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs, of the operator DNA hypersensitive to DNase I cleavage. The proximal, half-site of the inverted repeat partially overlaps with the predicted -35, consensus sequence of the dha promoter.
About this Structure
2IU4 is a Single protein structure of sequence from Lactococcus lactis with and as ligands. Active as Glucokinase, with EC number 2.7.1.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471
Page seeded by OCA on Sun Feb 3 10:38:14 2008
Categories: Glucokinase | Lactococcus lactis | Single protein | Baumann, U. | Christen, S. | Erni, B. | Srinivas, A. | HIQ | SO4 | Co-activator | Dihydroxyacetone | Kinase | Transferase
