2iw5

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[[Image:2iw5.gif|left|200px]]<br /><applet load="2iw5" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:2iw5.gif|left|200px]]<br /><applet load="2iw5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2iw5, resolution 2.57&Aring;" />
caption="2iw5, resolution 2.57&Aring;" />
'''STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE'''<br />
'''STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE'''<br />
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==About this Structure==
==About this Structure==
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2IW5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL, NH4, FAD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IW5 OCA].
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2IW5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NH4:'>NH4</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IW5 OCA].
==Reference==
==Reference==
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[[Category: transcription regulation]]
[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:37:23 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:38:53 2008''

Revision as of 08:38, 3 February 2008

Image:2iw5.gif

2iw5, resolution 2.57Å

Drag the structure with the mouse to rotate

STRUCTURAL BASIS FOR COREST-DEPENDENT DEMETHYLATION OF NUCLEOSOMES BY THE HUMAN LSD1 HISTONE DEMETHYLASE

Overview

Histone methylation regulates diverse chromatin-templated processes, including transcription. Many transcriptional corepressor complexes, contain lysine-specific demethylase 1 (LSD1) and CoREST that collaborate, to demethylate mono- and dimethylated H3-K4 of nucleosomes. Here, we, report the crystal structure of the LSD1-CoREST complex. LSD1-CoREST forms, an elongated structure with a long stalk connecting the catalytic domain, of LSD1 and the CoREST SANT2 domain. LSD1 recognizes a large segment of, the H3 tail through a deep, negatively charged pocket at the active site, and possibly a shallow groove on its surface. CoREST SANT2 interacts with, DNA. Disruption of the SANT2-DNA interaction diminishes CoREST-dependent, demethylation of nucleosomes by LSD1. The shape and dimension of, LSD1-CoREST suggest its bivalent binding to nucleosomes, allowing, efficient H3-K4 demethylation. This spatially separated, multivalent, nucleosome binding mode may apply to other chromatin-modifying enzymes, that generally contain multiple nucleosome binding modules.

About this Structure

2IW5 is a Protein complex structure of sequences from Homo sapiens with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase., Yang M, Gocke CB, Luo X, Borek D, Tomchick DR, Machius M, Otwinowski Z, Yu H, Mol Cell. 2006 Aug 4;23(3):377-87. PMID:16885027

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