2ix3
From Proteopedia
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| - | [[Image:2ix3.gif|left|200px]]<br /><applet load="2ix3" size=" | + | [[Image:2ix3.gif|left|200px]]<br /><applet load="2ix3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ix3, resolution 2.70Å" /> | caption="2ix3, resolution 2.70Å" /> | ||
'''STRUCTURE OF YEAST ELONGATION FACTOR 3'''<br /> | '''STRUCTURE OF YEAST ELONGATION FACTOR 3'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2IX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IX3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: translation]] | [[Category: translation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:39:13 2008'' |
Revision as of 08:39, 3 February 2008
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STRUCTURE OF YEAST ELONGATION FACTOR 3
Overview
Elongation factor eEF3 is an ATPase that, in addition to the two canonical, factors eEF1A and eEF2, serves an essential function in the translation, cycle of fungi. eEF3 is required for the binding of the, aminoacyl-tRNA-eEF1A-GTP ternary complex to the ribosomal A-site and has, been suggested to facilitate the clearance of deacyl-tRNA from the E-site., Here we present the crystal structure of Saccharomyces cerevisiae eEF3, showing that it consists of an amino-terminal HEAT repeat domain, followed, by a four-helix bundle and two ABC-type ATPase domains, with a, chromodomain inserted in ABC2. Moreover, we present the cryo-electron, microscopy structure of the ATP-bound form of eEF3 in complex with the, post-translocational-state 80S ribosome from yeast. eEF3 uses an entirely, new factor binding site near the ribosomal E-site, with the chromodomain, likely to stabilize the ribosomal L1 stalk in an open conformation, thus, allowing tRNA release.
About this Structure
2IX3 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of eEF3 and the mechanism of transfer RNA release from the E-site., Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R, Nature. 2006 Oct 12;443(7112):663-8. Epub 2006 Aug 23. PMID:16929303
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Categories: Saccharomyces cerevisiae | Single protein | Anand, M. | Andersen, C.B.F. | Andersen, G.R. | Balar, B. | Becker, T. | Beckmann, R. | Blau, M. | Boesen, T. | Halic, M. | Kinzy, T.G. | Mielke, T. | Pedersen, J.S. | Spahn, C.M.T. | SO4 | Acetylation | Atp-binding | Elongation factor | Nucleotide-binding | Phosphorylation | Protein biosynthesis | Rna-binding | Rrna-binding | Translation
