2ixh
From Proteopedia
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| - | [[Image:2ixh.jpg|left|200px]]<br /><applet load="2ixh" size=" | + | [[Image:2ixh.jpg|left|200px]]<br /><applet load="2ixh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2ixh, resolution 2.00Å" /> | caption="2ixh, resolution 2.00Å" /> | ||
'''RMLC P AERUGINOSA WITH DTDP-RHAMNOSE'''<br /> | '''RMLC P AERUGINOSA WITH DTDP-RHAMNOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with TRH as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Trh Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2IXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=TRH:'>TRH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Trh+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lipopolysaccharide biosynthesis]] | [[Category: lipopolysaccharide biosynthesis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:39:24 2008'' |
Revision as of 08:39, 3 February 2008
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RMLC P AERUGINOSA WITH DTDP-RHAMNOSE
Overview
The striking feature of carbohydrates is their constitutional, conformational and configurational diversity. Biology has harnessed this, diversity and manipulates carbohydrate residues in a variety of ways, one, of which is epimerization. RmlC catalyzes the epimerization of the C3' and, C5' positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming, dTDP-6-deoxy-L-lyxo-4-hexulose. RmlC is the third enzyme of the rhamnose, pathway, and represents a validated anti-bacterial drug target. Although, several structures of the enzyme have been reported, the mechanism and the, nature of the intermediates have remained obscure. Despite its relatively, small size (22 kDa), RmlC catalyzes four stereospecific proton transfers, and the substrate undergoes a major conformational change during the, course of the transformation. Here we report the structure of RmlC from, several organisms in complex with product and product mimics. We have, probed site-directed mutants by assay and by deuterium exchange. The, combination of structural and biochemical data has allowed us to assign, key residues and identify the conformation of the carbohydrate during, turnover. Clear knowledge of the chemical structure of RmlC reaction, intermediates may offer new opportunities for rational drug design.
About this Structure
2IXH is a Single protein structure of sequence from Pseudomonas aeruginosa with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation., Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH, J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787
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