2j12

From Proteopedia

Revision as of 08:40, 3 February 2008

AD37 FIBRE HEAD IN COMPLEX WITH CAR D1

Overview

Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and, adenovirus receptor (CAR), although the binding residues are not strictly, conserved. To understand this further, we determined the crystal, structures of canine adenovirus serotype 2 (CAV-2) and the human, adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and, 1.5A resolution, respectively. Structure comparison with the HAd12 fiber, head-CAR D1 complex showed that the overall topology of the interaction is, conserved but that the interfaces differ in number and identity of, interacting residues, shape complementarity, and degree of conformational, adaptation. Using surface plasmon resonance, we characterized the binding, affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We, found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved, interactions can have a minor contribution, whereas serotype-specific, interactions can be essential. These results are discussed in the light of, virus evolution and design of adenovirus vectors for gene transfer.

Disease

Known diseases associated with this structure: Adrenocortical tumor, somatic OMIM:[188830], Carney complex, type 1 OMIM:[188830], Myxoma, intracardiac OMIM:[188830], Pigmented adrenocortical disease, primary, 1 OMIM:[188830], Spastic paraplegia-7 OMIM:[602783], Thyroid carcinoma, papillary OMIM:[188830]

About this Structure

2J12 is a Protein complex structure of sequences from Homo sapiens and Human adenovirus type 49 with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor., Seiradake E, Lortat-Jacob H, Billet O, Kremer EJ, Cusack S, J Biol Chem. 2006 Nov 3;281(44):33704-16. Epub 2006 Aug 21. PMID:16923808

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