2j18
From Proteopedia
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| - | [[Image:2j18.jpg|left|200px]]<br /><applet load="2j18" size=" | + | [[Image:2j18.jpg|left|200px]]<br /><applet load="2j18" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2j18, resolution 1.75Å" /> | caption="2j18, resolution 1.75Å" /> | ||
'''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | '''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Br Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Br+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J18 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:40:30 2008'' |
Revision as of 08:40, 3 February 2008
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CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)
Overview
The X-ray crystallographic analysis of redox-active systems may be, complicated by photoreduction. Although radiolytic reduction by the, probing X-ray beam may be exploited to generate otherwise short-lived, reaction intermediates of metalloproteins, it is generally an undesired, feature. Here, the X-ray-induced reduction of the three heme proteins, myoglobin, cytochrome P450cam and chloroperoxidase has been followed by, on-line UV-Vis absorption spectroscopy. All three systems showed a very, rapid reduction of the heme iron. In chloroperoxidase the change of the, ionization state from ferric to ferrous heme is associated with a movement, of the heme-coordinating water molecule. The influence of the energy of, the incident X-ray photons and of the presence of scavengers on the, apparent reduction rate of ferric myoglobin crystals was analyzed.
About this Structure
2J18 is a Single protein structure of sequence from Leptoxyphium fumago with , , , and as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068
Page seeded by OCA on Sun Feb 3 10:40:30 2008
Categories: Chloride peroxidase | Leptoxyphium fumago | Single protein | Beitlich, T. | Kuhnel, K. | Schlichting, I. | Schulze-Briese, C. | Shoeman, R.L. | BR | HEM | MAN | MN | NAG | Chloride | Glycoprotein | Heme | Iron | Manganese | Metal-binding | Oxidoreductase | Peroxidase | Pyrrolidone carboxylic acid
