2dij
From Proteopedia
(New page: 200px<br /> <applet load="2dij" size="450" color="white" frame="true" align="right" spinBox="true" caption="2dij, resolution 2.6Å" /> '''COMPLEX OF A Y195F M...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2DIJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with CA and ADH as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1DIJ. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DIJ OCA]]. | + | 2DIJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with CA and ADH as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1DIJ. Active as [[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19]]. Structure known Active Sites: 16, 26, 36, CA1, CA2 and CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DIJ OCA]]. |
==Reference== | ==Reference== | ||
Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity., Strokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW, Biochemistry. 1996 Apr 2;35(13):4241-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8672460 8672460] | Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity., Strokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW, Biochemistry. 1996 Apr 2;35(13):4241-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8672460 8672460] | ||
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
| + | [[Category: Cyclomaltodextrin glucanotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dijkstra, B.W.]] | [[Category: Dijkstra, B.W.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:48:11 2007'' |
Revision as of 10:43, 30 October 2007
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COMPLEX OF A Y195F MUTANT CGTASE FROM B. CIRCULANS STRAIN 251 COMPLEXED WITH A MALTONONAOSE INHIBITOR AT PH 9.8 OBTAINED AFTER SOAKING THE CRYSTAL WITH ACARBOSE AND MALTOHEXAOSE
Overview
Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from, Bacillus circulans strain 251 were subjected to a double soaking, procedure, in which they were first soaked in a solution containing the, inhibitor acarbose and subsequently in a solution containing maltohexaose., The refined structure of the resulting protein-carbohydrate complex has, final crystallographic and free R-factors for data in the 8-2.6 angstrom, resolution range of 15.0% and 21.5%, respectively, and reveals that a new, inhibitor, composed of nine saccharide residues, is bound in the active, site. The first four residues correspond to acarbose and occupy the same, subsites near the catalytic residues as observed in the previously, reported acarbose-enzyme complex [Strokopytov et al. (1995) Biochemistry, ... [(full description)]
About this Structure
2DIJ is a [Single protein] structure of sequence from [Bacillus circulans] with CA and ADH as [ligands]. This structure superseeds the now removed PDB entry 1DIJ. Active as [Cyclomaltodextrin glucanotransferase], with EC number [2.4.1.19]. Structure known Active Sites: 16, 26, 36, CA1, CA2 and CAT. Full crystallographic information is available from [OCA].
Reference
Structure of cyclodextrin glycosyltransferase complexed with a maltononaose inhibitor at 2.6 angstrom resolution. Implications for product specificity., Strokopytov B, Knegtel RM, Penninga D, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW, Biochemistry. 1996 Apr 2;35(13):4241-9. PMID:8672460
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