2iyz
From Proteopedia
(New page: 200px<br /> <applet load="2iyz" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iyz, resolution 2.30Å" /> '''SHIKIMATE KINASE FR...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2IYZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with S3P and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IYZ OCA]]. | + | 2IYZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with S3P and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IYZ OCA]]. |
==Reference== | ==Reference== | ||
Mechanism of phosphoryl transfer catalyzed by shikimate kinase from Mycobacterium tuberculosis., Hartmann MD, Bourenkov GP, Oberschall A, Strizhov N, Bartunik HD, J Mol Biol. 2006 Dec 1;364(3):411-23. Epub 2006 Sep 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17020768 17020768] | Mechanism of phosphoryl transfer catalyzed by shikimate kinase from Mycobacterium tuberculosis., Hartmann MD, Bourenkov GP, Oberschall A, Strizhov N, Bartunik HD, J Mol Biol. 2006 Dec 1;364(3):411-23. Epub 2006 Sep 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17020768 17020768] | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
| + | [[Category: Shikimate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bartunik, H.D.]] | [[Category: Bartunik, H.D.]] | ||
| Line 33: | Line 34: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:48:53 2007'' |
Revision as of 10:44, 30 October 2007
|
SHIKIMATE KINASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH SHIKIMATE-3-PHOSPHATE AND ADP
Overview
The structural mechanism of the catalytic functioning of shikimate kinase, from Mycobacterium tuberculosis was investigated on the basis of a series, of high-resolution crystal structures corresponding to individual steps in, the enzymatic reaction. The catalytic turnover of shikimate and ATP into, the products shikimate-3-phosphate and ADP, followed by release of ADP, was studied in the crystalline environment. Based on a comparison of the, structural states before initiation of the reaction and immediately after, the catalytic step, we derived a structural model of the transition state, that suggests that phosphoryl transfer proceeds with inversion by an, in-line associative mechanism. The random sequential binding of shikimate, and nucleotides is associated with domain movements. We ... [(full description)]
About this Structure
2IYZ is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with S3P and ADP as [ligands]. Active as [Shikimate kinase], with EC number [2.7.1.71]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Mechanism of phosphoryl transfer catalyzed by shikimate kinase from Mycobacterium tuberculosis., Hartmann MD, Bourenkov GP, Oberschall A, Strizhov N, Bartunik HD, J Mol Biol. 2006 Dec 1;364(3):411-23. Epub 2006 Sep 5. PMID:17020768
Page seeded by OCA on Tue Oct 30 12:48:53 2007
Categories: Mycobacterium tuberculosis | Shikimate kinase | Single protein | Bartunik, H.D. | Bourenkov, G.P. | Hartmann, M.D. | Oberschall, A. | Strizhov, N. | ADP | S3P | Amino-acid biosynthesis | Aromatic amino acid biosynthesis | Atp-binding | Kinase | Magnesium | Metal-binding | Nucleotide-binding | P-loop kinase | Shikimate pathway | Transferase
