2iy5
From Proteopedia
(New page: 200px<br /> <applet load="2iy5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iy5, resolution 3.10Å" /> '''PHENYLALANYL-TRNA S...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2IY5 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]] with MG and FYA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IY5 OCA]]. | + | 2IY5 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]] with MG and FYA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Phenylalanine--tRNA_ligase Phenylalanine--tRNA ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IY5 OCA]]. |
==Reference== | ==Reference== | ||
The crystal structure of the ternary complex of phenylalanyl-tRNA synthetase with tRNAPhe and a phenylalanyl-adenylate analogue reveals a conformational switch of the CCA end., Moor N, Kotik-Kogan O, Tworowski D, Sukhanova M, Safro M, Biochemistry. 2006 Sep 5;45(35):10572-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16939209 16939209] | The crystal structure of the ternary complex of phenylalanyl-tRNA synthetase with tRNAPhe and a phenylalanyl-adenylate analogue reveals a conformational switch of the CCA end., Moor N, Kotik-Kogan O, Tworowski D, Sukhanova M, Safro M, Biochemistry. 2006 Sep 5;45(35):10572-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16939209 16939209] | ||
| + | [[Category: Phenylalanine--tRNA ligase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
| Line 37: | Line 38: | ||
[[Category: trna-binding]] | [[Category: trna-binding]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:49:13 2007'' |
Revision as of 10:44, 30 October 2007
|
PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH TRNA AND A PHENYLALANYL-ADENYLATE ANALOG
Overview
The crystal structure of the ternary complex of (alphabeta)(2), heterotetrameric phenylalanyl-tRNA synthetase (PheRS) from Thermus, thermophilus with cognate tRNA(Phe) and a nonhydrolyzable, phenylalanyl-adenylate analogue (PheOH-AMP) has been determined at 3.1 A, resolution. It reveals conformational changes in tRNA(Phe) induced by the, PheOH-AMP binding. The single-stranded 3' end exhibits a hairpin, conformation in contrast to the partial unwinding observed previously in, the binary PheRS.tRNA(Phe) complex. The CCA end orientation is stabilized, by extensive base-specific interactions of A76 and C75 with the protein, and by intra-RNA interactions of A73 with adjacent nucleotides. The, 4-amino group of the "bulged out" C75 is trapped by two negatively charged, residues of the beta ... [(full description)]
About this Structure
2IY5 is a [Protein complex] structure of sequences from [Thermus thermophilus] with MG and FYA as [ligands]. Active as [Phenylalanine--tRNA ligase], with EC number [6.1.1.20]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of the ternary complex of phenylalanyl-tRNA synthetase with tRNAPhe and a phenylalanyl-adenylate analogue reveals a conformational switch of the CCA end., Moor N, Kotik-Kogan O, Tworowski D, Sukhanova M, Safro M, Biochemistry. 2006 Sep 5;45(35):10572-83. PMID:16939209
Page seeded by OCA on Tue Oct 30 12:49:13 2007
Categories: Phenylalanine--tRNA ligase | Protein complex | Thermus thermophilus | Kotik-Kogan, O. | Moor, N. | Safro, M. | Sukhanova, M. | Tworowski, D. | FYA | MG | Aminoacyl-trna synthetase | Atp-binding | Class ii aminoacyl-trna synthetase | Helix-turn-helix motif | Ligase | Magnesium | Metal-binding | Nucleotide-binding | Phenylalanyl-trna synthetase | Protein biosynthesis | Rbd domin | Rna-binding | Sh3 domain | Trna-binding
