2c0r
From Proteopedia
(New page: 200px<br /> <applet load="2c0r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c0r, resolution 1.20Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2C0R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.52 2.6.1.52]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C0R OCA]]. | + | 2C0R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phosphoserine_transaminase Phosphoserine transaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.52 2.6.1.52]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C0R OCA]]. |
==Reference== | ==Reference== | ||
Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies., Kapetaniou EG, Thanassoulas A, Dubnovitsky AP, Nounesis G, Papageorgiou AC, Proteins. 2006 Jun 1;63(4):742-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16532449 16532449] | Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies., Kapetaniou EG, Thanassoulas A, Dubnovitsky AP, Nounesis G, Papageorgiou AC, Proteins. 2006 Jun 1;63(4):742-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16532449 16532449] | ||
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
| + | [[Category: Phosphoserine transaminase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kapetaniou, E.G.]] | [[Category: Kapetaniou, E.G.]] | ||
| Line 27: | Line 28: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:50:40 2007'' |
Revision as of 10:45, 30 October 2007
|
CRYSTAL STRUCTURE OF PHOSPHOSERINE AMINOTRANSFERASE FROM BACILLUS CIRCULANS VAR. ALKALOPHILUS AT PH 8.5
Overview
pH is one of the key parameters that affect the stability and function of, proteins. We have studied the effect of pH on the, pyridoxal-5'-phosphate-dependent enzyme phosphoserine aminotransferase, produced by the facultative alkaliphile Bacillus circulans ssp., alkalophilus using thermodynamic and crystallographic analysis. Enzymatic, activity assay showed that the enzyme has maximum activity at pH 9.0 and, relative activity less than 10% at pH 7.0. Differential scanning, calorimetry and circular dichroism experiments revealed variations in the, stability and denaturation profiles of the enzyme at different pHs. Most, importantly, release of pyridoxal-5'-phosphate and protein thermal, denaturation were found to occur simultaneously at pH 6.0 in contrast to, pH 8.5 where denaturation ... [(full description)]
About this Structure
2C0R is a [Single protein] structure of sequence from [Bacillus circulans] with PLP as [ligand]. Active as [Phosphoserine transaminase], with EC number [2.6.1.52]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Effect of pH on the structure and stability of Bacillus circulans ssp. alkalophilus phosphoserine aminotransferase: thermodynamic and crystallographic studies., Kapetaniou EG, Thanassoulas A, Dubnovitsky AP, Nounesis G, Papageorgiou AC, Proteins. 2006 Jun 1;63(4):742-53. PMID:16532449
Page seeded by OCA on Tue Oct 30 12:50:40 2007
Categories: Bacillus circulans | Phosphoserine transaminase | Single protein | Kapetaniou, E.G. | Papageorgiou, A.C. | PLP | Amino-acid biosynthesis | Aminotransferase | Phosphoserine aminotransferase | Pyridine serine biosynthesis | Pyridoxal phosphate | Pyridoxal-5'-phosphate | Pyridoxine biosynthesis | Serine biosynthesis | Transferase
