1gsk

From Proteopedia

Revision as of 10:46, 30 October 2007

CRYSTAL STRUCTURE OF COTA, AN ENDOSPORE COAT PROTEIN FROM BACILLUS SUBTILIS

Overview

Endospores produced by the Gram-positive soil bacterium Bacillus subtilis, are shielded by a proteinaceous coat formed by over 30 structural, components, which self-assemble into a lamellar inner coat and a thicker, striated electrodense outer coat. The 65-kDa CotA protein is an abundant, component of the outer coat layer. CotA is a highly thermostable laccase, assembly of which into the coat is required for spore resistance against, hydrogen peroxide and UV light. Here, we report the structure of CotA at, 1.7-A resolution, as determined by x-ray crystallography. This is the, first structure of an endospore coat component, and also the first, structure of a bacterial laccase. The overall fold of CotA comprises three, cupredoxin-like domains and includes one mononuclear and one trinuclear, ... [(full description)]

About this Structure

1GSK is a [Single protein] structure of sequence from [Bacillus subtilis] with CU, C2O, C1O and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of a bacterial endospore coat component. A laccase with enhanced thermostability properties., Enguita FJ, Martins LO, Henriques AO, Carrondo MA, J Biol Chem. 2003 May 23;278(21):19416-25. Epub 2003 Mar 13. PMID:12637519

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