4fua
From Proteopedia
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| - | [[Image:4fua.jpg|left|200px]]<br /><applet load="4fua" size=" | + | [[Image:4fua.jpg|left|200px]]<br /><applet load="4fua" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="4fua, resolution 2.43Å" /> | caption="4fua, resolution 2.43Å" /> | ||
'''L-FUCULOSE-1-PHOSPHATE ALDOLASE COMPLEX WITH PGH'''<br /> | '''L-FUCULOSE-1-PHOSPHATE ALDOLASE COMPLEX WITH PGH'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4FUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, SO4, BME and PGH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] Known structural/functional Sites: <scene name='pdbsite=ACT:The Active Center Is Defined By The Zn Ion, The Four Zn ...'>ACT</scene> and <scene name='pdbsite=PBS:Binding Site For The Substrate Phosphate Group'>PBS</scene>. Full crystallographic information is available from [http:// | + | 4FUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BME:'>BME</scene> and <scene name='pdbligand=PGH:'>PGH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] Known structural/functional Sites: <scene name='pdbsite=ACT:The+Active+Center+Is+Defined+By+The+Zn+Ion,+The+Four+Zn+...'>ACT</scene> and <scene name='pdbsite=PBS:Binding+Site+For+The+Substrate+Phosphate+Group'>PBS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FUA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc enzyme]] | [[Category: zinc enzyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:52:53 2008'' |
Revision as of 08:52, 3 February 2008
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L-FUCULOSE-1-PHOSPHATE ALDOLASE COMPLEX WITH PGH
Overview
The structure of L-fuculose-1-phosphate aldolase in a cubic crystal form, has been determined with and without the inhibitor, phosphoglycolohydroxamate at 2.4 and 2.7 angstrom (1 angstrom = 0.1 nm), resolution, respectively. This inhibitor mimics the enediolate transition, state of the substrate moiety dihydroxyacetone phosphate. The structures, showed that dihydroxyacetone phosphate ligates the zinc ion of this, metal-dependent class II aldolase with its hydroxyl and keto oxygen atoms, shifting Glu73 away from the zinc coordination sphere to a non-polar, environment. At this position Glu73 accepts a proton in the initial, reaction step, producing the enediolate which is then stabilized by the, zinc ion. The other substrate moiety L-lactaldehyde was modeled, because, no binding structure is yet available.
About this Structure
4FUA is a Single protein structure of sequence from Escherichia coli with , , and as ligands. Active as L-fuculose-phosphate aldolase, with EC number 4.1.2.17 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Catalytic mechanism of the metal-dependent fuculose aldolase from Escherichia coli as derived from the structure., Dreyer MK, Schulz GE, J Mol Biol. 1996 Jun 14;259(3):458-66. PMID:8676381
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