7a3h
From Proteopedia
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| - | [[Image:7a3h.gif|left|200px]]<br /><applet load="7a3h" size=" | + | [[Image:7a3h.gif|left|200px]]<br /><applet load="7a3h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="7a3h, resolution 0.95Å" /> | caption="7a3h, resolution 0.95Å" /> | ||
'''NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION'''<br /> | '''NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 7A3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens] with GOL and EOH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=ACI:Catalytic Acid/Base'>ACI</scene> and <scene name='pdbsite=NUC:Catalytic Nucleophile'>NUC</scene>. Full crystallographic information is available from [http:// | + | 7A3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens] with <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=EOH:'>EOH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=ACI:Catalytic+Acid/Base'>ACI</scene> and <scene name='pdbsite=NUC:Catalytic+Nucleophile'>NUC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: skew-boat]] | [[Category: skew-boat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:53:46 2008'' |
Revision as of 08:53, 3 February 2008
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NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION
Overview
The enzymatic hydrolysis of O-glycosidic linkages is one of the most, diverse and widespread reactions in nature and involves a classic, "textbook" enzyme mechanism. A multidisciplinary analysis of a, beta-glycoside hydrolase, the Cel5A from Bacillus agaradhaerens, is, presented in which the structures of each of the native, substrate, covalent-intermediate, and product complexes have been determined and, their interconversions analyzed kinetically, providing unprecedented, insights into the mechanism of this enzyme class. Substrate is bound in a, distorted 1S3 skew-boat conformation, thereby presenting the anomeric, carbon appropriately for nucleophilic attack as well as satisfying the, stereoelectronic requirements for an incipient oxocarbenium ion. Leaving, group departure results in the trapping of a covalent, alpha-glycosyl-enzyme intermediate in which the sugar adopts an, undistorted 4C1 conformation. Finally, hydrolysis of this intermediate, yields a product complex in which the sugar is bound in a partially, disordered mode, consistent with unfavorable interactions and low product, affinity.
About this Structure
7A3H is a Single protein structure of sequence from Bacillus agaradhaerens with and as ligands. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:9718293
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Categories: Bacillus agaradhaerens | Cellulase | Single protein | Brzozowski, A.M. | Dauter, M. | Davies, G.J. | Mackenzie, L. | Schulein, M. | Varrot, A. | Withers, S.G. | EOH | GOL | Cellulose degradation | Distortion | Endoglucanase | Glycoside hydrolase family 5 | Hydrolase | Michaelis complex | Skew-boat
