1ofl
From Proteopedia
(New page: 200px<br /> <applet load="1ofl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ofl, resolution 1.70Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OFL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pedobacter_heparinus Pedobacter heparinus]] with GLA, BGC, CA and MXZ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.4 4.2.2.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFL OCA]]. | + | 1OFL is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pedobacter_heparinus Pedobacter heparinus]] with GLA, BGC, CA and MXZ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_4.2.2.20 Transferred entry: 4.2.2.20]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.4 4.2.2.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFL OCA]]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Pedobacter heparinus]] | [[Category: Pedobacter heparinus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Transferred entry: 4.2.2.20]] | ||
[[Category: Cygler, M.]] | [[Category: Cygler, M.]] | ||
[[Category: Michel, G.]] | [[Category: Michel, G.]] | ||
| Line 25: | Line 26: | ||
[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:52:33 2007'' |
Revision as of 10:47, 30 October 2007
|
CRYSTAL STRUCTURE OF CHONDROITINASE B COMPLEXED TO DERMATAN SULFATE HEXASACCHARIDE
Overview
Chondroitinase B from Pedobacter heparinus is the only known enzyme, strictly specific for dermatan sulfate and is a widely used enzymatic tool, for the structural characterization of glycosaminoglycans. This, beta-helical polysaccharide lyase belongs to family PL-6 and cleaves the, beta(1,4) linkage of dermatan sulfate in a random manner, yielding, 4,5-unsaturated dermatan sulfate disaccharides as the product. The, previously reported structure of its complex with a dermatan sulfate, disaccharide product identified the -1 and -2 subsites of the catalytic, groove. We present here the structure of chondroitinase B complexed with, several dermatan sulfate and chondroitin sulfate oligosaccharides. In, particular, the soaking of chondroitinase B crystals with a dermatan, sulfate ... [(full description)]
About this Structure
1OFL is a [Single protein] structure of sequence from [Pedobacter heparinus] with GLA, BGC, CA and MXZ as [ligands]. Active as [Transferred entry: 4.2.2.20], with EC number [4.2.2.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery., Michel G, Pojasek K, Li Y, Sulea T, Linhardt RJ, Raman R, Prabhakar V, Sasisekharan R, Cygler M, J Biol Chem. 2004 Jul 30;279(31):32882-96. Epub 2004 May 21. PMID:15155751
Page seeded by OCA on Tue Oct 30 12:52:33 2007
Categories: Pedobacter heparinus | Single protein | Transferred entry: 4.2.2.20 | Cygler, M. | Michel, G. | BGC | CA | GLA | MXZ | Active site | Beta-elimination | Dematan sulfate | Lyase
