3bep
From Proteopedia
(New page: 200px<br /><applet load="3bep" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bep, resolution 1.920Å" /> '''Structure of a slid...) |
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==About this Structure== | ==About this Structure== | ||
| - | 3BEP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=5CY:'>5CY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=AC1:5cy Binding Site For Residue C 111'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEP OCA]. | + | 3BEP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=5CY:'>5CY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=AC1:5cy+Binding+Site+For+Residue+C+111'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 15:32:02 2008'' |
Revision as of 13:32, 6 February 2008
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Structure of a sliding clamp on DNA
Overview
The structure of the E. coli beta clamp polymerase processivity factor has, been solved in complex with primed DNA. Interestingly, the clamp directly, binds the DNA duplex and also forms a crystal contact with the ssDNA, template strand, which binds into the protein-binding pocket of the clamp., We demonstrate that these clamp-DNA interactions function in clamp, loading, perhaps by inducing the ring to close around DNA. Clamp binding, to template ssDNA may also serve to hold the clamp at a primed site after, loading or during switching of multiple factors on the clamp. Remarkably, the DNA is highly tilted as it passes through the beta ring. The, pronounced 22 degrees angle of DNA through beta may enable DNA to switch, between multiple factors bound to a single clamp simply by alternating, from one protomer of the ring to the other.
About this Structure
3BEP is a Protein complex structure of sequences from Escherichia coli with as ligand. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a sliding clamp on DNA., Georgescu RE, Kim SS, Yurieva O, Kuriyan J, Kong XP, O'Donnell M, Cell. 2008 Jan 11;132(1):43-54. PMID:18191219
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