1oao
From Proteopedia
(New page: 200px<br /> <applet load="1oao" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oao, resolution 1.90Å" /> '''NIZN[FE4S4] AND NIN...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OAO is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]] with SO4, FE2, BCT, ZN, NI, ACT, SF4, XCC, SX, FLH and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAO OCA]]. | + | 1OAO is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]] with SO4, FE2, BCT, ZN, NI, ACT, SF4, XCC, SX, FLH and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAO OCA]]. |
==Reference== | ==Reference== | ||
Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase., Darnault C, Volbeda A, Kim EJ, Legrand P, Vernede X, Lindahl PA, Fontecilla-Camps JC, Nat Struct Biol. 2003 Apr;10(4):271-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627225 12627225] | Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase., Darnault C, Volbeda A, Kim EJ, Legrand P, Vernede X, Lindahl PA, Fontecilla-Camps JC, Nat Struct Biol. 2003 Apr;10(4):271-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627225 12627225] | ||
| + | [[Category: Carbon-monoxide dehydrogenase (acceptor)]] | ||
[[Category: Moorella thermoacetica]] | [[Category: Moorella thermoacetica]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: wood/ljungdahl pathway]] | [[Category: wood/ljungdahl pathway]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:54:53 2007'' |
Revision as of 10:50, 30 October 2007
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NIZN[FE4S4] AND NINI[FE4S4] CLUSTERS IN CLOSED AND OPEN ALPHA SUBUNITS OF ACETYL-COA SYNTHASE/CARBON MONOXIDE DEHYDROGENASE
Overview
The crystal structure of the tetrameric alpha2beta2 acetyl-coenzyme A, synthase/carbon monoxide dehydrogenase from Moorella thermoacetica has, been solved at 1.9 A resolution. Surprisingly, the two alpha subunits, display different (open and closed) conformations. Furthermore, X-ray data, collected from crystals near the absorption edges of several metal ions, indicate that the closed form contains one Zn and one Ni at its active, site metal cluster (A-cluster) in the alpha subunit, whereas the open form, has two Ni ions at the corresponding positions. Alternative metal contents, at the active site have been observed in a recent structure of the same, protein in which A-clusters contained one Cu and one Ni, and in, reconstitution studies of a recombinant apo form of a related acetyl-CoA, ... [(full description)]
About this Structure
1OAO is a [Protein complex] structure of sequences from [Moorella thermoacetica] with SO4, FE2, BCT, ZN, NI, ACT, SF4, XCC, SX, FLH and GOL as [ligands]. Active as [Carbon-monoxide dehydrogenase (acceptor)], with EC number [1.2.99.2]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase., Darnault C, Volbeda A, Kim EJ, Legrand P, Vernede X, Lindahl PA, Fontecilla-Camps JC, Nat Struct Biol. 2003 Apr;10(4):271-9. PMID:12627225
Page seeded by OCA on Tue Oct 30 12:54:53 2007
Categories: Carbon-monoxide dehydrogenase (acceptor) | Moorella thermoacetica | Protein complex | Darnault, C. | Fontecilla-Camps, J.C. | Kim, E.J. | Legrand, P. | Lindahl, P.A. | Vernede, X. | Volbeda, A. | ACT | BCT | FE2 | FLH | GOL | NI | SF4 | SO4 | SX | XCC | ZN | Acetyl-coa formation | Electron transfer | Nickel | Oxidoreductase | Wood/ljungdahl pathway
