2p8v
From Proteopedia
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caption="2p8v, resolution 1.850Å" /> | caption="2p8v, resolution 1.850Å" /> | ||
'''Crystal structure of human Homer3 EVH1 domain'''<br /> | '''Crystal structure of human Homer3 EVH1 domain'''<br /> | ||
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| + | ==Overview== | ||
| + | T cell receptor (TCR) and costimulatory receptor (CD28) signals cooperate, in activating T cells, although understanding of how these pathways are, themselves regulated is incomplete. We found that Homer2 and Homer3, members of the Homer family of cytoplasmic scaffolding proteins, are, negative regulators of T cell activation. This is achieved through binding, of nuclear factor of activated T cells (NFAT) and by competing with, calcineurin. Homer-NFAT binding was also antagonized by active, serine-threonine kinase AKT, thereby enhancing TCR signaling via, calcineurin-dependent dephosphorylation of NFAT. This corresponded with, changes in cytokine expression and an increase in effector-memory T cell, populations in Homer-deficient mice, which also developed autoimmune-like, pathology. These results demonstrate a further means by which, costimulatory signals are regulated to control self-reactivity. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2P8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P8V OCA]. | + | 2P8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+122'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P8V OCA]. |
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| + | ==Reference== | ||
| + | NFAT binding and regulation of T cell activation by the cytoplasmic scaffolding Homer proteins., Huang GN, Huso DL, Bouyain S, Tu J, McCorkell KA, May MJ, Zhu Y, Lutz M, Collins S, Dehoff M, Kang S, Whartenby K, Powell J, Leahy D, Worley PF, Science. 2008 Jan 25;319(5862):476-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18218901 18218901] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bouyain, S.]] | [[Category: Bouyain, S.]] | ||
| - | [[Category: Huang, G.]] | + | [[Category: Huang, G.N.]] |
| - | [[Category: Leahy, D | + | [[Category: Leahy, D.]] |
[[Category: Tu, J.]] | [[Category: Tu, J.]] | ||
[[Category: Worley, P.F.]] | [[Category: Worley, P.F.]] | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:28:43 2008'' |
Revision as of 15:28, 6 February 2008
|
Crystal structure of human Homer3 EVH1 domain
Overview
T cell receptor (TCR) and costimulatory receptor (CD28) signals cooperate, in activating T cells, although understanding of how these pathways are, themselves regulated is incomplete. We found that Homer2 and Homer3, members of the Homer family of cytoplasmic scaffolding proteins, are, negative regulators of T cell activation. This is achieved through binding, of nuclear factor of activated T cells (NFAT) and by competing with, calcineurin. Homer-NFAT binding was also antagonized by active, serine-threonine kinase AKT, thereby enhancing TCR signaling via, calcineurin-dependent dephosphorylation of NFAT. This corresponded with, changes in cytokine expression and an increase in effector-memory T cell, populations in Homer-deficient mice, which also developed autoimmune-like, pathology. These results demonstrate a further means by which, costimulatory signals are regulated to control self-reactivity.
About this Structure
2P8V is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
NFAT binding and regulation of T cell activation by the cytoplasmic scaffolding Homer proteins., Huang GN, Huso DL, Bouyain S, Tu J, McCorkell KA, May MJ, Zhu Y, Lutz M, Collins S, Dehoff M, Kang S, Whartenby K, Powell J, Leahy D, Worley PF, Science. 2008 Jan 25;319(5862):476-81. PMID:18218901
Page seeded by OCA on Wed Feb 6 17:28:43 2008
Categories: Homo sapiens | Single protein | Bouyain, S. | Huang, G.N. | Leahy, D. | Tu, J. | Worley, P.F. | SO4 | Evh1 domain | Homer3 | Signaling protein
