2zb9
From Proteopedia
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caption="2zb9, resolution 2.25Å" /> | caption="2zb9, resolution 2.25Å" /> | ||
'''Crystal structure of TetR family transcription regulator SCO0332'''<br /> | '''Crystal structure of TetR family transcription regulator SCO0332'''<br /> | ||
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| + | ==Overview== | ||
| + | SCO0332 protein is a putative TetR-family transcriptional regulator from, Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was, determined at 2.25 A resolution by single-wavelength anomalous diffraction, (SAD) phasing using the S atoms of the native protein. SCO0332 contains a, helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and, forms a homodimer. The overall structure of SCO0332 shows significant, similarity to other TetR-family regulators. A systematic evolution of, ligands by exponential enrichment (SELEX) analysis indicated that SCO0332, has sequence-specific DNA-binding ability and determined the position of, the operator element of SCO0332 on the chromosomal DNA of S. coelicolor., An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds, to the operator sequence upstream of the sco0330 gene, which encodes a, putative short-chain oxidoreductase. These results suggest that SCO0332 is, a transcriptional repressor that regulates sco0330 gene expression. | ||
==About this Structure== | ==About this Structure== | ||
2ZB9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB9 OCA]. | 2ZB9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB9 OCA]. | ||
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| + | ==Reference== | ||
| + | Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18219120 18219120] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces coelicolor]] | [[Category: Streptomyces coelicolor]] | ||
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[[Category: transcription regulator]] | [[Category: transcription regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:29:29 2008'' |
Revision as of 15:29, 6 February 2008
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Crystal structure of TetR family transcription regulator SCO0332
Overview
SCO0332 protein is a putative TetR-family transcriptional regulator from, Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was, determined at 2.25 A resolution by single-wavelength anomalous diffraction, (SAD) phasing using the S atoms of the native protein. SCO0332 contains a, helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and, forms a homodimer. The overall structure of SCO0332 shows significant, similarity to other TetR-family regulators. A systematic evolution of, ligands by exponential enrichment (SELEX) analysis indicated that SCO0332, has sequence-specific DNA-binding ability and determined the position of, the operator element of SCO0332 on the chromosomal DNA of S. coelicolor., An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds, to the operator sequence upstream of the sco0330 gene, which encodes a, putative short-chain oxidoreductase. These results suggest that SCO0332 is, a transcriptional repressor that regulates sco0330 gene expression.
About this Structure
2ZB9 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:18219120
Page seeded by OCA on Wed Feb 6 17:29:29 2008
