1w18
From Proteopedia
(New page: 200px<br /> <applet load="1w18" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w18, resolution 2.50Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1W18 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gluconacetobacter_diazotrophicus Gluconacetobacter diazotrophicus]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W18 OCA]]. | + | 1W18 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Gluconacetobacter_diazotrophicus Gluconacetobacter diazotrophicus]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Levansucrase Levansucrase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W18 OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus., Martinez-Fleites C, Ortiz-Lombardia M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernandez L, Davies GJ, Biochem J. 2005 Aug 15;390(Pt 1):19-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15869470 15869470] | Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus., Martinez-Fleites C, Ortiz-Lombardia M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernandez L, Davies GJ, Biochem J. 2005 Aug 15;390(Pt 1):19-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15869470 15869470] | ||
[[Category: Gluconacetobacter diazotrophicus]] | [[Category: Gluconacetobacter diazotrophicus]] | ||
| + | [[Category: Levansucrase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davies, G.J.]] | [[Category: Davies, G.J.]] | ||
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[[Category: glycosyltransferase]] | [[Category: glycosyltransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:56:30 2007'' |
Revision as of 10:51, 30 October 2007
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CRYSTAL STRUCTURE OF LEVANSUCRASE FROM GLUCONACETOBACTER DIAZOTROPHICUS
Overview
The endophytic Gram-negative bacterium Gluconacetobacter diazotrophicus, SRT4 secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10), which converts sucrose into fructooligosaccharides and levan. The enzyme, is included in GH (glycoside hydrolase) family 68 of the sequence-based, classification of glycosidases. The three-dimensional structure of LsdA, has been determined by X-ray crystallography at a resolution of 2.5 A (1, A=0.1 nm). The structure was solved by molecular replacement using the, homologous Bacillus subtilis (Bs) levansucrase (Protein Data Bank, accession code 1OYG) as a search model. LsdA displays a five-bladed, beta-propeller architecture, where the catalytic residues that are, responsible for sucrose hydrolysis are perfectly superimposable with the, ... [(full description)]
About this Structure
1W18 is a [Single protein] structure of sequence from [Gluconacetobacter diazotrophicus] with SO4 as [ligand]. Active as [Levansucrase], with EC number [2.4.1.10]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus., Martinez-Fleites C, Ortiz-Lombardia M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernandez L, Davies GJ, Biochem J. 2005 Aug 15;390(Pt 1):19-27. PMID:15869470
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