2ix9
From Proteopedia
(New page: 200px<br /> <applet load="2ix9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ix9, resolution 1.70Å" /> '''RESPECTIVE ROLE OF ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IX9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]] with SO4, CXS and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IX9 OCA]]. | + | 2IX9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]] with SO4, CXS and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Feruloyl_esterase Feruloyl esterase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.73 3.1.1.73]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IX9 OCA]]. |
==Reference== | ==Reference== | ||
Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17027758 17027758] | Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17027758 17027758] | ||
[[Category: Aspergillus niger]] | [[Category: Aspergillus niger]] | ||
| + | [[Category: Feruloyl esterase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Benoit, I.]] | [[Category: Benoit, I.]] | ||
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[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:56:48 2007'' |
Revision as of 10:52, 30 October 2007
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RESPECTIVE ROLE OF PROTEIN FOLDING AND GLYCOSYLATION IN THE THERMAL STABILITY OF RECOMBINANT FERULOYL ESTERASE A
Overview
The thermal stability of four molecular forms (native, refolded, glycosylated, non-glycosylated) of feruloyl esterase A (FAEA) was studied., From the most to the least thermo-resistant, the four molecular species, ranked as follows: (i) glycosylated form produced native, (ii), non-glycosylated form produced native, (iii) non-glycosylated form, produced as inclusion bodies and refolded, and (iv) glycosylated form, produced native chemically denatured and then refolded. On the basis of, these results and of crystal structure data, we discuss the respective, importance of protein folding and glycosylation in the thermal stability, of recombinant FAEA.
About this Structure
2IX9 is a [Single protein] structure of sequence from [Aspergillus niger] with SO4, CXS and EDO as [ligands]. Active as [Feruloyl esterase], with EC number [3.1.1.73]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A., Benoit I, Asther M, Sulzenbacher G, Record E, Marmuse L, Parsiegla G, Gimbert I, Asther M, Bignon C, FEBS Lett. 2006 Oct 30;580(25):5815-21. Epub 2006 Sep 27. PMID:17027758
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