2ixs
From Proteopedia
(New page: 200px<br /> <applet load="2ixs" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ixs, resolution 2.00Å" /> '''STRUCTURE OF SDAI R...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IXS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_diastaticus Streptomyces diastaticus]] with SO4, EPE and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IXS OCA]]. | + | 2IXS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_diastaticus Streptomyces diastaticus]] with SO4, EPE and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IXS OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces diastaticus]] | [[Category: Streptomyces diastaticus]] | ||
| + | [[Category: Type II site-specific deoxyribonuclease]] | ||
[[Category: Grazulis, S.]] | [[Category: Grazulis, S.]] | ||
[[Category: Huber, R.]] | [[Category: Huber, R.]] | ||
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[[Category: sdai]] | [[Category: sdai]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:56:59 2007'' |
Revision as of 10:52, 30 October 2007
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STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE
Overview
Rare-cutting restriction enzymes are important tools in genome analysis., We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the, cleavage site). Unlike orthodox Type IIP enzymes, which are single domain, proteins, the SdaI monomer is composed of two structural domains. The N, domain contains a classical winged helix-turn-helix (wHTH) DNA binding, motif, while the C domain shows a typical restriction endonuclease fold., The active site of SdaI is located within the C domain and represents a, variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence, specificity are clustered on the recognition helix of the wHTH motif at, the N domain. The modular architecture of SdaI, wherein one domain, ... [(full description)]
About this Structure
2IXS is a [Single protein] structure of sequence from [Streptomyces diastaticus] with SO4, EPE and TRS as [ligands]. Active as [Type II site-specific deoxyribonuclease], with EC number [3.1.21.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970
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