2ris

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2ris" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ris, resolution 1.600&Aring;" /> '''Crystal structure o...)
Line 4: Line 4:
==Overview==
==Overview==
-
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone, 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5, kDa peptide in a recombinant Escherichia coli strain. The recombinant, protein was purified to apparent homogeneity by two chromatographic steps, and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone, 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1), min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa, in line with a homodimer structure. The protein was crystallized in its, apoform. Soaking yielded crystals in complex with the substrate ribulose, 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7, angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate, synthase of E. coli for molecular replacement. Structural comparison with, the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed, a hitherto unknown conformation of the essential acidic active-site loop.
+
Reinterpretation of the space-group symmetry is reported for two crystal, structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate, synthase (PDB codes 1tks and 1tku). The two structures reported in space, group P1 with a dimer in the asymmetric unit can be described as, C-centered monoclinic structures with one subunit in the asymmetric unit.
==About this Structure==
==About this Structure==
Line 10: Line 10:
==Reference==
==Reference==
-
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328619 15328619]
+
Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase., Le Trong I, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):219-20. Epub 2008, Jan 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18219123 18219123]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 22: Line 22:
[[Category: riboflavin biosynthesis]]
[[Category: riboflavin biosynthesis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 11:00:29 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:18:38 2008''

Revision as of 06:18, 13 February 2008

Image:2ris.jpg

2ris, resolution 1.600Å

Drag the structure with the mouse to rotate

Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of candida albicans- alternate interpretation

Overview

Reinterpretation of the space-group symmetry is reported for two crystal, structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate, synthase (PDB codes 1tks and 1tku). The two structures reported in space, group P1 with a dimer in the asymmetric unit can be described as, C-centered monoclinic structures with one subunit in the asymmetric unit.

About this Structure

2RIS is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase., Le Trong I, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):219-20. Epub 2008, Jan 16. PMID:18219123

Page seeded by OCA on Wed Feb 13 08:18:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ris"
Personal tools