2riu

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(New page: 200px<br /><applet load="2riu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2riu, resolution 1.700&Aring;" /> '''Alternative models ...)
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==Overview==
==Overview==
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A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone, 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5, kDa peptide in a recombinant Escherichia coli strain. The recombinant, protein was purified to apparent homogeneity by two chromatographic steps, and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone, 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1), min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa, in line with a homodimer structure. The protein was crystallized in its, apoform. Soaking yielded crystals in complex with the substrate ribulose, 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7, angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate, synthase of E. coli for molecular replacement. Structural comparison with, the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed, a hitherto unknown conformation of the essential acidic active-site loop.
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Reinterpretation of the space-group symmetry is reported for two crystal, structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate, synthase (PDB codes 1tks and 1tku). The two structures reported in space, group P1 with a dimer in the asymmetric unit can be described as, C-centered monoclinic structures with one subunit in the asymmetric unit.
==About this Structure==
==About this Structure==
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2RIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=5RP:'>5RP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:5rp Binding Site For Residue A 701'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RIU OCA].
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2RIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=5RP:'>5RP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:5rp+Binding+Site+For+Residue+A+701'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RIU OCA].
==Reference==
==Reference==
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Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328619 15328619]
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Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase., Le Trong I, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):219-20. Epub 2008, Jan 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18219123 18219123]
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: riboflavin biosynthesis]]
[[Category: riboflavin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 11:00:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:18:39 2008''

Revision as of 06:18, 13 February 2008

Image:2riu.jpg

2riu, resolution 1.700Å

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Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase- alternate interpreation

Overview

Reinterpretation of the space-group symmetry is reported for two crystal, structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate, synthase (PDB codes 1tks and 1tku). The two structures reported in space, group P1 with a dimer in the asymmetric unit can be described as, C-centered monoclinic structures with one subunit in the asymmetric unit.

About this Structure

2RIU is a Single protein structure of sequence from Candida albicans with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase., Le Trong I, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):219-20. Epub 2008, Jan 16. PMID:18219123

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