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2pbj
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(New page: 200px<br /><applet load="2pbj" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pbj, resolution 2.8Å" /> '''GSH-heme bound micros...)
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Revision as of 06:20, 13 February 2008
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GSH-heme bound microsomal prostaglandin E synthase
Overview
Prostaglandin E2 synthase (PGES) catalyzes the isomerization of PGH2 to, PGE2. PGES type 2 (mPGES-2) is a membrane-associated enzyme, whose, N-terminal section is apparently inserted into the lipid bilayer. Both, intact and N-terminal truncated enzymes have been isolated and have, similar catalytic activity. The recombinant N-terminal truncated enzyme, purified from Escherichia coli HB101 grown in LB medium containing, delta-aminolevulinate and Fe(NO3)3 has a red color, while the same enzyme, purified from the same E. coli grown in minimal medium has no color. The, red-colored enzyme has been characterized by mass, fluorescence, and EPR, spectroscopies and X-ray crystallography. The enzyme is found to contain, bound glutathione (GSH) and heme. GSH binds to the active site with six, H-bonds, while a heme is complexed with bound GSH forming a S-Fe, coordination bond with no polar interaction with mPGES-2. There is a large, open space between the heme and the protein, where a PGH2 might be able to, bind. The heme dissociation constant is 0.53 microM, indicating that, mPGES-2 has relatively strong heme affinity. Indeed, expression of mPGES-2, in E. coli stimulates heme biosynthesis. Although mPGES-2 has been, reported to be a GSH-independent PGES, the crystal structure and sequence, analysis indicate that mPGES-2 is a GSH-binding protein. The GSH-heme, complex-bound enzyme (mPGES-2h) catalyzes formation of, 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid and malondialdehyde, from PGH2, but not formation of PGE2. The following kinetic parameters at, 37 degrees C were determined: KM = 56 microM, kcat = 63 s-1, and kcat/KM =, 1.1 x 10(6) M-1 s-1. They suggest that mPGES-2h has significant catalytic, activity for PGH2 degradation. It is possible that both GSH-heme, complex-free and -bound enzymes are present in the same tissues. mPGES-2, in heme-rich liver is most likely to become the form of mPGES-2h and might, be involved in degradation reactions similar to that of cytochrome P450., Since mPGES-2 is an isomerase and mPGES-2h is a lyase, mPGES-2 cannot, simply be classified into one of six classes set by the International, Union of Biochemistry and Molecular Biology.
About this Structure
2PBJ is a Single protein structure of sequence from Macaca fascicularis with , and as ligands. Active as Prostaglandin-E synthase, with EC number 5.3.99.3 Known structural/functional Sites: , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
PGH2 degradation pathway catalyzed by GSH-heme complex bound microsomal prostaglandin E2 synthase type 2: the first example of a dual-function enzyme., Yamada T, Takusagawa F, Biochemistry. 2007 Jul 17;46(28):8414-24. Epub 2007 Jun 22. PMID:17585783
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