3bs6
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(New page: 200px<br /><applet load="3bs6" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bs6, resolution 1.800Å" /> '''1.8 Angstrom crysta...)
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Revision as of 06:23, 13 February 2008
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1.8 Angstrom crystal structure of the periplasmic domain of the membrane insertase YidC
Overview
In bacteria the biogenesis of inner membrane proteins requires targeting, and insertion factors such as the signal recognition particle and the, Sec-translocon. YidC is an essential membrane protein involved in the, insertion of inner membrane proteins together with the Sec-translocon, but, also as a separate entity. YidC of Escherichia coli is a member of the, conserved YidC (in bacteria)/Oxa1 (in mitochondria)/Alb3 (in chloroplasts), protein family and contains six transmembrane segments and a large, periplasmic domain (P1). We determined the crystal structure of the, periplasmic domain of YidC from E.coli (P1D) at 1.8A resolution. The, structure of P1D shows the conserved ss-supersandwich-fold of carbohydrate, binding proteins and an a-helical linker region at the C-terminus that, packs against the ss-supersandwich by a highly conserved interface. P1D, exhibits an elongated cleft of similar architecture as found in the, structural homologs. However the electrostatic properties and molecular, details of the cleft make it unlikely to interact with carbohydrate, substrates. The cleft in P1D is occupied by a polyethylene glycol molecule, suggesting an elongated peptide or acyl chain as a natural ligand. The, region of P1D previously reported to interact with SecF maps to a surface, area in the vicinity of the cleft. The conserved C-terminal region of the, P1 domain was reported to be essential for the membrane insertase function, of YidC. The analysis of this region suggests a role in membrane, interaction and/or in the regulation of YidC interaction with binding, partners.
About this Structure
3BS6 is a Single protein structure of sequence from Escherichia coli with , , , and as ligands. Known structural/functional Sites: , , , , , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
The crystal structure of the periplasmic domain of the Escherichia coli membrane protein insertase YidC contains a conserved substrate binding cleft., Ravaud S, Stjepanovic G, Wild K, Sinning I, J Biol Chem. 2008 Jan 30;. PMID:18234665
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