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1cq0
From Proteopedia
(New page: 200px<br /> <applet load="1cq0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cq0" /> '''SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/...) |
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'''SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B ''''<br /> | '''SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B ''''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CQ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1CQ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: solution structure]] | [[Category: solution structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:36:34 2008'' |
Revision as of 13:36, 15 February 2008
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SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '
Contents |
Overview
Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively) are novel hypothalamic neuropeptides encoded by a single, mRNA transcript; they stimulate food intake. We have determined the, three-dimensional solution structure of human hypocretin-2/orexin-B using, two-dimensional 1H-NMR data and dynamical simulated annealing, calculations. On the basis of NOEs, 3JHNalpha coupling constants and, hydrogen-deuterium exchange rates together with chemical shift indices, human hypocretin-2/orexin-B was deduced to consist of two alpha-helices, connected with a short linker in both H2O and 30% trifluoroethanol, solutions. The helical axis of helix I is oriented about 60-80 degrees, relative to helix II. Hybrid distance geometry and simulated-annealing, protocols were used to generate an ensemble of 30 structures with no, constraint violations greater than 0.03 nm for distances and 3 degrees for, angles. In addition, human hypocretin-2/orexin-B shares a similar, secondary-structural motif with human neuropeptide Y. This result can form, the basis for further study on ligand-receptor recognition of human orexin, receptors.
Disease
Known diseases associated with this structure: Narcolepsy OMIM:[602358], Porphyria variegata OMIM:[600923]
About this Structure
1CQ0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of a new hypothalamic neuropeptide, human hypocretin-2/orexin-B., Lee JH, Bang E, Chae KJ, Kim JY, Lee DW, Lee W, Eur J Biochem. 1999 Dec;266(3):831-9. PMID:10583376
Page seeded by OCA on Fri Feb 15 15:36:34 2008
Categories: Homo sapiens | Single protein | Bang, E.J. | Chae, K.J. | Lee, D.W. | Lee, K.H. | Lee, W. | Human hcrt-2/ox-b | Neuropeptide | Nmr | Obesity | Solution structure
