1cvw
From Proteopedia
(New page: 200px<br /> <applet load="1cvw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cvw, resolution 2.28Å" /> '''CRYSTAL STRUCTURE O...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1cvw. | + | [[Image:1cvw.jpg|left|200px]]<br /><applet load="1cvw" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1cvw" size=" | + | |
caption="1cvw, resolution 2.28Å" /> | caption="1cvw, resolution 2.28Å" /> | ||
'''CRYSTAL STRUCTURE OF ACTIVE SITE-INHIBITED HUMAN COAGULATION FACTOR VIIA (DES-GLA)'''<br /> | '''CRYSTAL STRUCTURE OF ACTIVE SITE-INHIBITED HUMAN COAGULATION FACTOR VIIA (DES-GLA)'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1CVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and ANS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Coagulation_factor_VIIa Coagulation factor VIIa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.21 3.4.21.21] Full crystallographic information is available from [http:// | + | 1CVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ANS:'>ANS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Coagulation_factor_VIIa Coagulation factor VIIa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.21 3.4.21.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVW OCA]. |
==Reference== | ==Reference== | ||
| Line 31: | Line 30: | ||
[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:37:35 2008'' |
Revision as of 13:37, 15 February 2008
|
CRYSTAL STRUCTURE OF ACTIVE SITE-INHIBITED HUMAN COAGULATION FACTOR VIIA (DES-GLA)
Contents |
Overview
Factor VIIa (FVIIa) is a crucial haemostatic protease consisting of four, distinct domains termed the Gla, epidermal growth factor-1 (EGF-1), EGF-2, and protease domains (from N- to C-terminus). The crystal structure of, human FVIIa inhibited at the active site with 1, 5-dansyl-Glu-Gly-Arg-chloromethyl ketone and lacking the Gla domain has, been solved to a resolution of 2.28 A. The EGF-2 and protease domains were, well resolved, whereas no electron density for the EGF-1 domain was, observed, suggesting a flexible arrangement or disorder within the, crystal. Superposition of the protease domain of the present structure, with that previously resolved in the tissue factor (TF)/FVIIai complex, revealed that although overall the domain structures are similar, the, EGF-2 domain is rotated by 7.5 degrees relative to the protease domain on, binding TF. A single cleavage in the protease domain was found, between, Arg315 and Lys316 (chymotrypsin numbering 170C-170D) in a FVII-specific, insertion loop: this cleavage appeared to be essential for, crystallisation. Insertion of the heavy chain N-terminal Ile153 is, essentially identical in the two structures, as is the geometry of the, active site residues and the inhibitor C-terminal arginine residue. Some, differences are seen in the cleaved loop, but changes in TF-contact, residues are generally minor. This structure supports the hypothesis that, TF binding enables spatial domain arrangements in the flexible FVIIa, molecule necessary for procoagulant function and furthermore that active, site occupancy induces FVIIa active conformation via N-terminal insertion.
Disease
Known diseases associated with this structure: Factor VII deficiency OMIM:[227500], Myocardial infarction, decreased susceptibility to OMIM:[227500]
About this Structure
1CVW is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Coagulation factor VIIa, with EC number 3.4.21.21 Full crystallographic information is available from OCA.
Reference
Crystal structure of active site-inhibited human coagulation factor VIIa (des-Gla)., Kemball-Cook G, Johnson DJ, Tuddenham EG, Harlos K, J Struct Biol. 1999 Oct;127(3):213-23. PMID:10544046
Page seeded by OCA on Fri Feb 15 15:37:35 2008
