1d8v
From Proteopedia
(New page: 200px<br /> <applet load="1d8v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d8v" /> '''THE RESTRAINED AND MINIMIZED AVERAGE NMR ST...) |
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'''THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.'''<br /> | '''THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1D8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http:// | + | 1D8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8V OCA]. |
==Reference== | ==Reference== | ||
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[[Category: single chain]] | [[Category: single chain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:39:00 2008'' |
Revision as of 13:39, 15 February 2008
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THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.
Overview
We present the solution structure of MAP30, a plant protein with anti-HIV, and anti-tumor activities. Structural analysis and subsequent biochemical, assays lead to several novel discoveries. First, MAP30 acts like a DNA, glycosylase/apurinic (ap) lyase, an additional activity distinct from its, known RNA N-glycosidase activity toward the 28S rRNA. Glycosylase/ap lyase, activity explains MAP30's apparent inhibition of the HIV-1 integrase, MAP30's ability to irreversibly relax supercoiled DNA, and may be an, alternative cytotoxic pathway that contributes to MAP30's, anti-HIV/anti-tumor activities. Second, two distinct, but contiguous, subsites are responsible for MAP30's glycosylase/ap lyase activity. Third, Mn2+ and Zn2+ interact with negatively charged surfaces next to the, catalytic sites, facilitating DNA substrate binding instead of directly, participating in catalysis.
About this Structure
1D8V is a Single protein structure of sequence from Momordica charantia. Full crystallographic information is available from OCA.
Reference
Solution structure of anti-HIV-1 and anti-tumor protein MAP30: structural insights into its multiple functions., Wang YX, Neamati N, Jacob J, Palmer I, Stahl SJ, Kaufman JD, Huang PL, Huang PL, Winslow HE, Pommier Y, Wingfield PT, Lee-Huang S, Bax A, Torchia DA, Cell. 1999 Nov 12;99(4):433-42. PMID:10571185
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