1deg
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caption="1deg, resolution 2.9Å" /> | caption="1deg, resolution 2.9Å" /> | ||
'''THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE'''<br /> | '''THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DEG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DEG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: calcium-binding protein]] | [[Category: calcium-binding protein]] | ||
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Revision as of 13:39, 15 February 2008
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THE LINKER OF DES-GLU84 CALMODULIN IS BENT AS SEEN IN THE CRYSTAL STRUCTURE
Contents |
Overview
The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been, refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM, the central helix is fully extended, and the molecule is dumbbell shaped., In contrast, the deletion of Glu-84 causes a bend of 95 degrees in the, linker region of the central helix at Ile-85. However, EF-hand domains 1, and 2 (lobe 1,2) do not touch lobe 3,4. The length, by alpha-carbon, separation, of des-Glu84-CaM is 56 A; that of native CaM is 64 A. The, shape of des-Glu84-CaM is similar to that of native CaM, as it is bound to, the target peptide of myosin light-chain kinase. This result supports the, proposal that the linker region of the central helix of CaM functions as a, flexible tether.
Disease
Known diseases associated with this structure: Cavernous malformations of CNS and retina OMIM:[604214], Cerebral cavernous malformations-1 OMIM:[604214], Hyperkeratotic cutaneous capillary-venous malformations associated with cerebral capillary malformations OMIM:[604214], Leukemia, acute T-cell lymphoblastic OMIM:[603025], Leukemia, acute myeloid OMIM:[603025]
About this Structure
1DEG is a Single protein structure of sequence from Bos taurus with as ligand. Full crystallographic information is available from OCA.
Reference
The linker of des-Glu84-calmodulin is bent., Raghunathan S, Chandross RJ, Cheng BP, Persechini A, Sobottka SE, Kretsinger RH, Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6869-73. PMID:8341712
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