1e0s

From Proteopedia

Revision as of 13:41, 15 February 2008

SMALL G PROTEIN ARF6-GDP

Overview

Arf6 is an isoform of Arf that localizes at the periphery of the cell, where it has an essential role in endocytotic pathways. Its function does, not overlap with that of Arf1, although the two proteins share, approximately 70% sequence identity and they have switch regions, whose, conformation depends on the nature of the guanine nucleotide, with almost, identical sequences. The crystal structure of Arf6-GDP at 2.3 A shows that, it has a conformation similar to that of Arf1-GDP, which cannot bind, membranes with high affinity. Significantly, the switch regions of Arf6, deviate by 2-5 A from those of Arf1. These differences are a consequence, of the shorter N-terminal linker of Arf6 and of discrete sequence changes, between Arf6 and Arf1. Mutational analysis shows that one of the positions, which differs between Arf1 and Arf6 affects the configuration of the, nucleotide binding site and thus the nucleotide binding properties of the, Arf variant. Altogether, our results provide a structural basis for, understanding how Arf1 and Arf6 can be distinguished by their guanine, nucleotide exchange factors and suggest a model for the, nucleotide/membrane cycle of Arf6.

About this Structure

1E0S is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.

Reference

Structure of Arf6-GDP suggests a basis for guanine nucleotide exchange factors specificity., Menetrey J, Macia E, Pasqualato S, Franco M, Cherfils J, Nat Struct Biol. 2000 Jun;7(6):466-9. PMID:10881192

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