2bvd
From Proteopedia
(New page: 200px<br /> <applet load="2bvd" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bvd, resolution 1.60Å" /> '''HOW FAMILY 26 GLYCO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BVD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]] with ISX as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BVD OCA]]. | + | 2BVD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]] with ISX as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BVD OCA]]. |
==Reference== | ==Reference== | ||
How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A., Taylor EJ, Goyal A, Guerreiro CI, Prates JA, Money VA, Ferry N, Morland C, Planas A, Macdonald JA, Stick RV, Gilbert HJ, Fontes CM, Davies GJ, J Biol Chem. 2005 Sep 23;280(38):32761-7. Epub 2005 Jun 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15987675 15987675] | How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A., Taylor EJ, Goyal A, Guerreiro CI, Prates JA, Money VA, Ferry N, Morland C, Planas A, Macdonald JA, Stick RV, Gilbert HJ, Fontes CM, Davies GJ, J Biol Chem. 2005 Sep 23;280(38):32761-7. Epub 2005 Jun 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15987675 15987675] | ||
| + | [[Category: Cellulase]] | ||
[[Category: Clostridium thermocellum]] | [[Category: Clostridium thermocellum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lichenase]] | [[Category: lichenase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:02:24 2007'' |
Revision as of 10:57, 30 October 2007
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HOW FAMILY 26 GLYCOSIDE HYDROLASES ORCHESTRATE CATALYSIS ON DIFFERENT POLYSACCHARIDES. STRUCTURE AND ACTIVITY OF A CLOSTRIDIUM THERMOCELLUM LICHENASE, CTLIC26A
Overview
One of the most intriguing features of the 90 glycoside hydrolase families, (GHs) is the range of specificities displayed by different members of the, same family, whereas the catalytic apparatus and mechanism are often, invariant. Family GH26 predominantly comprises beta-1,4 mannanases;, however, a bifunctional Clostridium thermocellum GH26 member (hereafter, CtLic26A) displays a markedly different specificity. We show that CtLic26A, is a lichenase, specific for mixed (Glcbeta1,4Glcbeta1,4Glcbeta1,3)n, oligo- and polysaccharides, and displays no activity on manno-configured, substrates or beta-1,4-linked homopolymers of glucose or xylose. The, three-dimensional structure of the native form of CtLic26A has been solved, at 1.50-A resolution, revealing a characteristic (beta/alpha)8 barrel ... [(full description)]
About this Structure
2BVD is a [Single protein] structure of sequence from [Clostridium thermocellum] with ISX as [ligand]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A., Taylor EJ, Goyal A, Guerreiro CI, Prates JA, Money VA, Ferry N, Morland C, Planas A, Macdonald JA, Stick RV, Gilbert HJ, Fontes CM, Davies GJ, J Biol Chem. 2005 Sep 23;280(38):32761-7. Epub 2005 Jun 28. PMID:15987675
Page seeded by OCA on Tue Oct 30 13:02:24 2007
Categories: Cellulase | Clostridium thermocellum | Single protein | Davies, G.J. | Ferry, N. | Fontes, C.M.G.A. | Gilbert, H.J. | Goyal, A. | Guerreiro, C.I.P.D. | Macdonald, J.A. | Money, V.A. | Morland, C. | Planas, A. | Prates, J.A.M. | Stick, R.V. | Taylor, E.J. | ISX | Beta-1 4 beta-1 3 glucanase | Glycoside hydrolase family 26 | Lichenase
