1fgu
From Proteopedia
(New page: 200px<br /> <applet load="1fgu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fgu, resolution 2.50Å" /> '''SSDNA-BINDING DOMAI...) |
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| - | [[Image:1fgu. | + | [[Image:1fgu.jpg|left|200px]]<br /><applet load="1fgu" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1fgu, resolution 2.50Å" /> | caption="1fgu, resolution 2.50Å" /> | ||
'''SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A'''<br /> | '''SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1FGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ssdna-binding protein]] | [[Category: ssdna-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:47:41 2008'' |
Revision as of 13:47, 15 February 2008
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SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A
Overview
Although structures of single-stranded (ss)DNA-binding proteins (SSBs), have been reported with and without ssDNA, the mechanism of ssDNA binding, in eukarya remains speculative. Here we report a 2.5 Angstroms structure, of the ssDNA-binding domain of human replication protein A (RPA), (eukaryotic SSB), for which we previously reported a structure in complex, with ssDNA. A comparison of free and bound forms of RPA revealed that, ssDNA binding is associated with a major reorientation between, and, significant conformational changes within, the structural, modules--OB-folds--which comprise the DNA-binding domain. Two OB-folds, whose tandem orientation was stabilized by the presence of DNA, adopted, multiple orientations in its absence. Within the OB-folds, extended loops, implicated in DNA binding significantly changed conformation in the, absence of DNA. Analysis of intermolecular contacts suggested the, possibility that other RPA molecules and/or other proteins could compete, with DNA for the same binding site. Using this mechanism, protein-protein, interactions can regulate, and/or be regulated by DNA binding. Combined, with available biochemical data, this structure also suggested a dynamic, model for the DNA-binding mechanism.
About this Structure
1FGU is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the major single-stranded DNA-binding domain of replication protein A suggests a dynamic mechanism for DNA binding., Bochkareva E, Belegu V, Korolev S, Bochkarev A, EMBO J. 2001 Feb 1;20(3):612-8. PMID:11157767
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