User:Olivia Cheng/Sandbox 1

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--[[User:Olivia Cheng|Olivia Cheng]] 23:30, 27 March 2012 (IST)This is my sandbox!
 
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Outer Surface Protein B ([[OspB]]) is a component
 
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[[Hemoglobin]]
 
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== OspB Interaction with Fab of H6831 ==
 
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<Structure load='1RJL' size='500' frame='true' align='right' caption='[http://proteopedia.com/wiki/index.php/1rjl 1RJL] with Chain C in purple and Chains A, B, and D white' scene='User:Olivia_Cheng/Sandbox_1/1rjl_color/1'/>
 
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Click here to revert to the <scene name='User:Olivia_Cheng/Sandbox_1/1rjl_color/1'>original model</scene>.
 
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<scene name='User:Olivia_Cheng/Sandbox_1/1rjl_chainc_transparentabd/2'>Transparent</scene>
 
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OspB consists of <scene name='User:Olivia_Cheng/Sandbox_1/1rjl_chainc/5'>chain C</scene>.
 
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<scene name='User:Olivia_Cheng/Sandbox_1/1rjl_chainc_lys253/3'>Lys-253 on Chain C</scene>
 
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H6831 along with CB2 are a monoclonal antibodies.
 
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H6831 appears to have a bactericidal effect on OspB in the absence of complement proteins.
 
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<!--Monoclonal antibodies LINK
 
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[http://www.proteopedia.org/wiki/index.php/Monoclonal_antibody Monoclonal antibodies]
 
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== Potential Catalytic Triad ==
 
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<!--
 
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1P4P link
 
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<Structure load='1P4P' size='500' frame='true' align='right' caption='[http://proteopedia.com/wiki/index.php/1p4p 1P4P]' scene='' />
 
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Proposed Mechanism
 
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<scene name='User:Olivia_Cheng/Sandbox_1/1p4p_lys253/1'>Lys-253</scene>
 
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There are 3 residues that exist on the eliminated B-sheet of OspB that highly resemble the catalytic triad on [http://en.wikipedia.org/wiki/Serine_protease Serine proteasoccur.
 
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== References ==
 
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Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL. Structural investigation of ''Borrelia burgdorferi'' OspB, a bactericidal Fab target. J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713683 15713683] doi:[http://www.jbc.org/content/280/17/17363 10.1074/jbc.M412842200]
 
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Connolly, S. The Versatile Roles of Antibodies in Borrelia Infections
 
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Katona, L. A Bactericidal Monoclonal Antibody Elicits a Change in Its Antigen, OspB of Borrelia burgdorferi, That Can Be Detected by Limited Proteolysis
 

Revision as of 21:50, 27 March 2012

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Olivia Cheng

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