1bag
From Proteopedia
(New page: 200px<br /> <applet load="1bag" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bag, resolution 2.5Å" /> '''ALPHA-AMYLASE FROM B...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BAG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BAG OCA]]. | + | 1BAG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Sites: CA1, CA2 and CA3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BAG OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose., Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H, J Mol Biol. 1998 Mar 27;277(2):393-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9514750 9514750] | Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose., Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H, J Mol Biol. 1998 Mar 27;277(2):393-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9514750 9514750] | ||
| + | [[Category: Alpha-amylase]] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: maltopentaose]] | [[Category: maltopentaose]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:04:45 2007'' |
Revision as of 11:00, 30 October 2007
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ALPHA-AMYLASE FROM BACILLUS SUBTILIS COMPLEXED WITH MALTOPENTAOSE
Overview
The X-ray crystal structure of a catalytic-site mutant EQ208, [Glu208-->Gln] of alpha-amylase from Bacillus subtilis cocrystallized with, maltopentaose (G5) and acarbose has been determined by multiple, isomorphous replacement at 2.5 A resolution. Restrained crystallographic, refinement has resulted in an R-factor of 19.8% in the 7.0 to 2.5 A, resolution range. EQ208 consists of three domains containing a, (beta/alpha)8-barrel as observed in other alpha-amylases. Clear connected, density corresponding to a pentasaccharide was observed, which was, considered as the G5 molecule based on the high affinity of EQ208 for G5, that could replace pre-bound acarbose or a possible transglycosylation, product of acarbose. The conformation around the third, alpha-(1,4)-glucosidic bond makes a sharp ... [(full description)]
About this Structure
1BAG is a [Single protein] structure of sequence from [Bacillus subtilis] with CA as [ligand]. Active as [Alpha-amylase], with EC number [3.2.1.1]. Structure known Active Sites: CA1, CA2 and CA3. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose., Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H, J Mol Biol. 1998 Mar 27;277(2):393-407. PMID:9514750
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