Maltose-binding protein
From Proteopedia
| Line 5: | Line 5: | ||
==3D structures of maltose-binding protein== | ==3D structures of maltose-binding protein== | ||
| - | [[1llx]] - MBP<br /> | + | [[1llx]], [[1jw4]], [[1lls]] - MBP<br /> |
| - | [[3sey]], [[3sex]], [[3sew]], [[3sev]], [[3seu]], [[3set]], [[3ses]], [[3ser]], [[2v93]], [[1zkb]], [[1n3x]], [[1peb ]] | + | [[3sey]], [[3sex]], [[3sew]], [[3sev]], [[3seu]], [[3set]], [[3ses]], [[3ser]], [[2v93]], [[1zkb]], [[1n3x]], [[1peb ]], [[1mbp]], [[1mpc]], [[3kjt]] - MBP (mutant)<br /> |
[[2klf]], [[2d21]] – MBP – NMR<br /> | [[2klf]], [[2d21]] – MBP – NMR<br /> | ||
[[1ziu]] – MBP (mutant) + Ni<br /> | [[1ziu]] – MBP (mutant) + Ni<br /> | ||
[[1zjl]] – MBP (mutant) + Zn<br /> | [[1zjl]] – MBP (mutant) + Zn<br /> | ||
| - | [[1zmg]] - MBP (mutant) + Cu | + | [[1zmg]] - MBP (mutant) + Cu<br /> |
| + | [[2xd2]] – SpMBP – ''Streptococcus pneumoniae'' | ||
'''MBP complex with saccharide''' | '''MBP complex with saccharide''' | ||
| - | [[1n3w]], [[1nl5]] - MBP (mutant) + maltose<br /> | + | [[1n3w]], [[1nl5]], [[1mpd]], [[1iud]], [[1jvx]], [[1jvy]] - MBP (mutant) + maltose<br /> |
| + | [[1anf]], [[1jw5]] - MBP + maltose<br /> | ||
| + | [[3mbp]] - MBP + maltotriose<br /> | ||
| + | [[1ez9]] - MBP + maltodextrin<br /> | ||
[[3hpi]] – MBP (mutant) + sucrose<br /> | [[3hpi]] – MBP (mutant) + sucrose<br /> | ||
| + | [[1lax]] - MBP (mutant) + glucose<br /> | ||
[[2h25]] - MBP + β-cyclodextrin<br /> | [[2h25]] - MBP + β-cyclodextrin<br /> | ||
| - | [[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol | + | [[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol<br /> |
| + | [[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br /> | ||
| + | [[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br /> | ||
| + | [[2xd3]] – SpMBP + maltopentaose | ||
'''MBP complex with protein''' | '''MBP complex with protein''' | ||
| Line 25: | Line 33: | ||
[[3puv]], [[3puw]], [[3pux]] – MBP + maltose transporter complex + ADP <br /> | [[3puv]], [[3puw]], [[3pux]] – MBP + maltose transporter complex + ADP <br /> | ||
[[3rlf]] - MBP + maltose transporter complex + Mg-AMPPNP<br /> | [[3rlf]] - MBP + maltose transporter complex + Mg-AMPPNP<br /> | ||
| - | [[1svx]] – MBP + ankyrin repeat protein OFF7 | + | [[1svx]] – MBP + ankyrin repeat protein OFF7<br /> |
| + | [[1nmu]] - MBP + ribosomal protein L30<br /> | ||
| + | [[1t0k]] - MBP + ribosomal protein L30 + RNA | ||
| + | |||
'''MBP fused to other proteins''' | '''MBP fused to other proteins''' | ||
| Line 42: | Line 53: | ||
[[1r6z]]– MBP/argonaute 2<br /> | [[1r6z]]– MBP/argonaute 2<br /> | ||
[[1hsj]] - MBP/accessary regulator protein R<br /> | [[1hsj]] - MBP/accessary regulator protein R<br /> | ||
| - | [[3osq]], [[3osr]] – MBP/Green fluorescent protein | + | [[3osq]], [[3osr]] – MBP/Green fluorescent protein<br /> |
| + | [[2vgq]] – MBP/IPS-1 card<br /> | ||
| + | [[3ehs]] – MBP/CRFR1<br /> | ||
| + | [[3eht]], [[3ehu]] – MBP/CRFR1 + CRF<br /> | ||
| + | [[3h3g]] – MBP/parathyroid hormone + PTH-related protein<br /> | ||
| + | [[3l2j]] - MBP/parathyroid hormone receptor<br /> | ||
| + | [[3ior]], [[3iot]], [[3iou]], [[3iov]], [[3iow]], [[3io4]], [[3io6]] – MBP/Huntington disease protein<br /> | ||
| + | [[3o3u]] – MBP/Advanced glycosylation end product-specific receptor<br /> | ||
| + | [[3n94]] – MBP/pituitary adenylate cyclase 1 receptor | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 09:33, 29 March 2012
Maltose-binding protein (MBP) is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose.
3D structures of maltose-binding protein
1llx, 1jw4, 1lls - MBP
3sey, 3sex, 3sew, 3sev, 3seu, 3set, 3ses, 3ser, 2v93, 1zkb, 1n3x, 1peb , 1mbp, 1mpc, 3kjt - MBP (mutant)
2klf, 2d21 – MBP – NMR
1ziu – MBP (mutant) + Ni
1zjl – MBP (mutant) + Zn
1zmg - MBP (mutant) + Cu
2xd2 – SpMBP – Streptococcus pneumoniae
MBP complex with saccharide
1n3w, 1nl5, 1mpd, 1iud, 1jvx, 1jvy - MBP (mutant) + maltose
1anf, 1jw5 - MBP + maltose
3mbp - MBP + maltotriose
1ez9 - MBP + maltodextrin
3hpi – MBP (mutant) + sucrose
1lax - MBP (mutant) + glucose
2h25 - MBP + β-cyclodextrin
1fqa, 1fqb, 1fqc, 1fqd - MBP + maltotetraitol
1elj – MBP + glucose – Pyrococcus furiosus
3jyr – MBP + α-acarbose – Salmonella enterica
2xd3 – SpMBP + maltopentaose
MBP complex with protein
3pgf – MBP (mutant) + synthetic antigen binder
2r6g - MBP + maltose transporter complex
3puv, 3puw, 3pux – MBP + maltose transporter complex + ADP
3rlf - MBP + maltose transporter complex + Mg-AMPPNP
1svx – MBP + ankyrin repeat protein OFF7
1nmu - MBP + ribosomal protein L30
1t0k - MBP + ribosomal protein L30 + RNA
MBP fused to other proteins
3hst – MBP/RNase H domain
3mq9 – MBP/bone marrow stromal antigen 2
3lbs - MBP/renin receptor + maltose
3lc8 - MBP/renin receptor
3csb – MBP/monobody YSX1
3csg - MBP/monobody YS1
2obg - MBP/monobody MBP-74
3f5f - MBP/Heparan sulfate 2-O-sulfotransferase
3g7v, 3g7w - MBP/islet amyloid polypeptide
2zxt, 3a3c - MBP/mitochondrial intermembrane space import and assembly protein 40
1ytv - MBP/vasopressin receptor
1r6z– MBP/argonaute 2
1hsj - MBP/accessary regulator protein R
3osq, 3osr – MBP/Green fluorescent protein
2vgq – MBP/IPS-1 card
3ehs – MBP/CRFR1
3eht, 3ehu – MBP/CRFR1 + CRF
3h3g – MBP/parathyroid hormone + PTH-related protein
3l2j - MBP/parathyroid hormone receptor
3ior, 3iot, 3iou, 3iov, 3iow, 3io4, 3io6 – MBP/Huntington disease protein
3o3u – MBP/Advanced glycosylation end product-specific receptor
3n94 – MBP/pituitary adenylate cyclase 1 receptor
