1i01
From Proteopedia
(New page: 200px<br /> <applet load="1i01" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i01, resolution 2.6Å" /> '''CRYSTAL STRUCTURE OF...) |
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caption="1i01, resolution 2.6Å" /> | caption="1i01, resolution 2.6Å" /> | ||
'''CRYSTAL STRUCTURE OF BETA-KETOACYL [ACYL CARRIER PROTEIN] REDUCTASE FROM E. COLI.'''<br /> | '''CRYSTAL STRUCTURE OF BETA-KETOACYL [ACYL CARRIER PROTEIN] REDUCTASE FROM E. COLI.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I01 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1I01 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb90_1.html Fatty Acid Synthase]]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Full crystallographic information is available from [http:// | + | 1I01 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1I01 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb90_1.html Fatty Acid Synthase]]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I01 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rossman fold]] | [[Category: rossman fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:58:57 2008'' |
Revision as of 13:58, 15 February 2008
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CRYSTAL STRUCTURE OF BETA-KETOACYL [ACYL CARRIER PROTEIN] REDUCTASE FROM E. COLI.
Overview
The structure of beta-ketoacyl-[acyl carrier protein] reductase (FabG), from Escherichia coli was determined via the multiwavelength anomalous, diffraction technique using a selenomethionine-labeled crystal containing, 88 selenium sites in the asymmetric unit. The comparison of the E. coli, FabG structure with the homologous Brassica napus FabG.NADP(+) binary, complex reveals that cofactor binding causes a substantial conformational, change in the protein. This conformational change puts all three, active-site residues (Ser 138, Tyr 151, and Lys 155) into their active, configurations and provides a structural mechanism for allosteric, communication between the active sites in the homotetramer. FabG exhibits, negative cooperative binding of NADPH, and this effect is enhanced by the, presence of acyl carrier protein (ACP). NADPH binding also increases the, affinity and decreases the maximum binding of ACP to FabG. Thus, unlike, other members of the short-chain dehydrogenase/reductase superfamily, FabG, undergoes a substantial conformational change upon cofactor binding that, organizes the active-site triad and alters the affinity of the other, substrate-binding sites in the tetrameric enzyme.
About this Structure
1I01 is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1I01 with [Fatty Acid Synthase]. Active as [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 Full crystallographic information is available from OCA.
Reference
Structure of beta-ketoacyl-[acyl carrier protein] reductase from Escherichia coli: negative cooperativity and its structural basis., Price AC, Zhang YM, Rock CO, White SW, Biochemistry. 2001 Oct 30;40(43):12772-81. PMID:11669613 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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