1i7z
From Proteopedia
(New page: 200px<br /> <applet load="1i7z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i7z, resolution 2.3Å" /> '''ANTIBODY GNC92H2 BOU...) |
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| - | [[Image:1i7z. | + | [[Image:1i7z.jpg|left|200px]]<br /><applet load="1i7z" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1i7z, resolution 2.3Å" /> | caption="1i7z, resolution 2.3Å" /> | ||
'''ANTIBODY GNC92H2 BOUND TO LIGAND'''<br /> | '''ANTIBODY GNC92H2 BOUND TO LIGAND'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I7Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_and_homo_sapiens Mus musculus and homo sapiens] with COC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1I7Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus_and_homo_sapiens Mus musculus and homo sapiens] with <scene name='pdbligand=COC:'>COC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:59:53 2008'' |
Revision as of 13:59, 15 February 2008
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ANTIBODY GNC92H2 BOUND TO LIGAND
Contents |
Overview
Murine monoclonal antibody GNC92H2 was elicited by active immunization, with a cocaine immunoconjugate and binds free cocaine with excellent, specificity and moderate affinity. Improvement of affinity, as well as, humanization of GNC92H2, would be advantageous in immunopharmacotherapy, for cocaine addiction, and for emergency cases of drug overdose. Toward, this end, the crystal structure of an engineered murine-human chimeric Fab, of GNC92H2 complexed with cocaine was determined at 2.3 A resolution., Structural analysis reveals a binding pocket with high shape and charge, complementarity to the cocaine framework, which explains the specificity, for cocaine, as opposed to the pharmacologically inactive cocaine, metabolites. Importantly, the structure provides a foundation for, mutagenesis to enhance the binding affinity for cocaine and potent cocaine, derivatives, such as cocaethylene, and for additional humanization of the, antibody.
Disease
Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]
About this Structure
1I7Z is a Protein complex structure of sequences from Mus musculus and homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a cocaine-binding antibody., Larsen NA, Zhou B, Heine A, Wirsching P, Janda KD, Wilson IA, J Mol Biol. 2001 Aug 3;311(1):9-15. PMID:11469854
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