This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jgu
From Proteopedia
(New page: 200px<br /> <applet load="1jgu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jgu, resolution 1.8Å" /> '''STRUCTURAL BASIS FOR...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1jgu. | + | [[Image:1jgu.jpg|left|200px]]<br /><applet load="1jgu" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1jgu" size=" | + | |
caption="1jgu, resolution 1.8Å" /> | caption="1jgu, resolution 1.8Å" /> | ||
'''STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4'''<br /> | '''STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with OH and HBC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1JGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=OH:'>OH</scene> and <scene name='pdbligand=HBC:'>HBC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGU OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 23: | ||
[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:07:18 2008'' |
Revision as of 14:07, 15 February 2008
|
STRUCTURAL BASIS FOR DISFAVORED ELIMINATION REACTION IN CATALYTIC ANTIBODY 1D4
Overview
Murine antibody 1D4 selectively catalyzes a highly disfavored, beta-elimination reaction. Crystal structures of unliganded 1D4 and 1D4 in, complex with a transition-state analog (TSA) have elucidated a possible, general base mode of catalysis. The structures of the unliganded and, liganded Fabs were determined to 1.80 and 1.85 A resolution, respectively., The structure of the complex reveals a binding pocket with high shape, complementarity to the TSA, which is recruited to coerce the substrate, into the sterically demanding, eclipsed conformation that is required for, catalysis. A histidine residue and two water molecules are likely involved, in the catalysis. The structure supports either a concerted E2 or stepwise, E1cB-like mechanism for elimination. Finally, the liganded 1D4 structure, shows minor conformational rearrangements in CDR H2, indicative of, induced-fit binding of the hapten. 1D4 has pushed the boundaries of, antibody-mediated catalysis into the realm of disfavored reactions and, hence, represents an important milestone in the development of this, technology.
About this Structure
1JGU is a Single protein structure of sequence from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for a disfavored elimination reaction in catalytic antibody 1D4., Larsen NA, Heine A, Crane L, Cravatt BF, Lerner RA, Wilson IA, J Mol Biol. 2001 Nov 16;314(1):93-102. PMID:11724535
Page seeded by OCA on Fri Feb 15 16:07:18 2008
Categories: Mus musculus | Single protein | Crane, L. | Cravatt, B.F. | Heine, A. | Larsen, N.A. | Lerner, R.A. | Wilson, I.A. | HBC | OH | Igg fold
