1jmt
From Proteopedia
(New page: 200px<br /> <applet load="1jmt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jmt, resolution 2.20Å" /> '''X-ray Structure of ...) |
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caption="1jmt, resolution 2.20Å" /> | caption="1jmt, resolution 2.20Å" /> | ||
'''X-ray Structure of a Core U2AF65/U2AF35 Heterodimer'''<br /> | '''X-ray Structure of a Core U2AF65/U2AF35 Heterodimer'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JMT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HEZ as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JMT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HEZ:'>HEZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JMT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rrm]] | [[Category: rrm]] | ||
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Revision as of 14:08, 15 February 2008
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X-ray Structure of a Core U2AF65/U2AF35 Heterodimer
Overview
U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes, the 3' splice site and recruits the U2 snRNP to the branch point. The, X-ray structure of the human core U2AF heterodimer, consisting of the, U2AF35 central domain and a proline-rich region of U2AF65, has been, determined at 2.2 A resolution. The structure reveals a novel, protein-protein recognition strategy, in which an atypical RNA recognition, motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via, reciprocal "tongue-in-groove" tryptophan residues. Complementary, biochemical experiments demonstrate that the core U2AF heterodimer binds, RNA, and that the interacting tryptophan side chains are essential for, U2AF dimerization. Atypical RRMs in other splicing factors may serve as, protein-protein interaction motifs elsewhere during spliceosome assembly.
About this Structure
1JMT is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer., Kielkopf CL, Rodionova NA, Green MR, Burley SK, Cell. 2001 Sep 7;106(5):595-605. PMID:11551507
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