1jpl
From Proteopedia
(New page: 200px<br /> <applet load="1jpl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jpl, resolution 2.40Å" /> '''GGA3 VHS domain com...) |
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caption="1jpl, resolution 2.40Å" /> | caption="1jpl, resolution 2.40Å" /> | ||
'''GGA3 VHS domain complexed with C-terminal peptide from cation-independent mannose 6-phosphate receptor'''<br /> | '''GGA3 VHS domain complexed with C-terminal peptide from cation-independent mannose 6-phosphate receptor'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JPL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1JPL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JPL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein-peptide complex; vhs domain; dxxll sorting signal]] | [[Category: protein-peptide complex; vhs domain; dxxll sorting signal]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:08:31 2008'' |
Revision as of 14:08, 15 February 2008
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GGA3 VHS domain complexed with C-terminal peptide from cation-independent mannose 6-phosphate receptor
Contents |
Overview
Specific sorting signals direct transmembrane proteins to the compartments, of the endosomal-lysosomal system. Acidic-cluster-dileucine signals, present within the cytoplasmic tails of sorting receptors, such as the, cation-independent and cation-dependent mannose-6-phosphate receptors, are, recognized by the GGA (Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding) proteins. The VHS (Vps27p, Hrs and STAM), domains of the GGA proteins are responsible for the highly specific, recognition of these acidic-cluster-dileucine signals. Here we report the, structures of the VHS domain of human GGA3 complexed with signals from, both mannose-6-phosphate receptors. The signals bind in an extended, conformation to helices 6 and 8 of the VHS domain. The structures, highlight an Asp residue separated by two residues from a dileucine, sequence as critical recognition elements. The side chains of the, Asp-X-X-Leu-Leu sequence interact with subsites consisting of one, electropositive and two shallow hydrophobic pockets, respectively. The, rigid spatial alignment of the three binding subsites leads to high, specificity.
Disease
Known diseases associated with this structure: Hepatocellular carcinoma OMIM:[147280]
About this Structure
1JPL is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains., Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH, Nature. 2002 Feb 21;415(6874):933-7. PMID:11859375
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