1k3y
From Proteopedia
(New page: 200px<br /> <applet load="1k3y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k3y, resolution 1.30Å" /> '''Crystal Structure A...) |
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caption="1k3y, resolution 1.30Å" /> | caption="1k3y, resolution 1.30Å" /> | ||
'''Crystal Structure Analysis of human Glutathione S-transferase with S-hexyl glutatione and glycerol at 1.3 Angstrom'''<br /> | '''Crystal Structure Analysis of human Glutathione S-transferase with S-hexyl glutatione and glycerol at 1.3 Angstrom'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GTX and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http:// | + | 1K3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GTX:'>GTX</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:11:19 2008'' |
Revision as of 14:11, 15 February 2008
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Crystal Structure Analysis of human Glutathione S-transferase with S-hexyl glutatione and glycerol at 1.3 Angstrom
Overview
Cytosolic glutathione S-transferases (GSTs) play a critical role in, xenobiotic binding and metabolism, as well as in modulation of oxidative, stress. Here, the high-resolution X-ray crystal structures of homodimeric, human GSTA1-1 in the apo form and in complex with S-hexyl glutathione (two, data sets) are reported at 1.8, 1.5, and 1.3A respectively. At this level, of resolution, distinct conformations of the alkyl chain of S-hexyl, glutathione are observed, reflecting the nonspecific nature of the, hydrophobic substrate binding site (H-site). Also, an extensive network of, ordered water, including 75 discrete solvent molecules, traverses the open, subunit-subunit interface and connects the glutathione binding sites in, each subunit. In the highest-resolution structure, three glycerol moieties, lie within this network and directly connect the amino termini of the, glutathione molecules. A search for ligand binding sites with the docking, program Molecular Operating Environment identified the ordered water, network binding site, lined mainly with hydrophobic residues, suggesting, an extended ligand binding surface for nonsubstrate ligands, the so-called, ligandin site. Finally, detailed comparison of the structures reported, here with previously published X-ray structures reveal a possible reaction, coordinate for ligand-dependent conformational changes in the active site, and the C-terminus.
About this Structure
1K3Y is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Full crystallographic information is available from OCA.
Reference
1.3-A resolution structure of human glutathione S-transferase with S-hexyl glutathione bound reveals possible extended ligandin binding site., Le Trong I, Stenkamp RE, Ibarra C, Atkins WM, Adman ET, Proteins. 2002 Sep 1;48(4):618-27. PMID:12211029
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