1kem
From Proteopedia
(New page: 200px<br /> <applet load="1kem" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kem, resolution 2.20Å" /> '''CATALYTIC ANTIBODY ...) |
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| - | [[Image:1kem. | + | [[Image:1kem.jpg|left|200px]]<br /><applet load="1kem" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1kem, resolution 2.20Å" /> | caption="1kem, resolution 2.20Å" /> | ||
'''CATALYTIC ANTIBODY 28B4 FAB FRAGMENT'''<br /> | '''CATALYTIC ANTIBODY 28B4 FAB FRAGMENT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KEM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1KEM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KEM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sulfide oxidation]] | [[Category: sulfide oxidation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:13:05 2008'' |
Revision as of 14:13, 15 February 2008
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CATALYTIC ANTIBODY 28B4 FAB FRAGMENT
Overview
The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of, antibody 28B4 complexed with hapten have been solved at 2.2-angstrom and, 1.9-angstrom resolution, respectively. To our knowledge, these structures, are the highest resolution catalytic antibody structures to date and, provide insight into the molecular mechanism of this antibody-catalyzed, monooxygenation reaction. Specifically, the data suggest that entropic, restriction plays a fundamental role in catalysis through the precise, alignment of the thioether substrate and oxidant. The antibody active site, also stabilizes developing charge on both sulfur and periodate in the, transition state via cation-pi and electrostatic interactions, respectively. In addition to demonstrating that the active site of, antibody 28B4 does indeed reflect the mechanistic information programmed, in the aminophosphonic acid hapten, these high-resolution structures, provide a basis for enhancing turnover rates through mutagenesis and, improved hapten design.
About this Structure
1KEM is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Insights into antibody catalysis: structure of an oxygenation catalyst at 1.9-angstrom resolution., Hsieh-Wilson LC, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5363-7. PMID:8643580
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