1lq0
From Proteopedia
(New page: 200px<br /> <applet load="1lq0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lq0, resolution 2.20Å" /> '''CRYSTAL STRUCTURE O...) |
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caption="1lq0, resolution 2.20Å" /> | caption="1lq0, resolution 2.20Å" /> | ||
'''CRYSTAL STRUCTURE OF HUMAN CHITOTRIOSIDASE AT 2.2 ANGSTROM RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF HUMAN CHITOTRIOSIDASE AT 2.2 ANGSTROM RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LQ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1LQ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQ0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: gaucher]] | [[Category: gaucher]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:19:52 2008'' |
Revision as of 14:19, 15 February 2008
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CRYSTAL STRUCTURE OF HUMAN CHITOTRIOSIDASE AT 2.2 ANGSTROM RESOLUTION
Overview
Chitin hydrolases have been identified in a variety of organisms ranging, from bacteria to eukaryotes. They have been proposed to be possible, targets for the design of novel chemotherapeutics against human pathogens, such as fungi and protozoan parasites as mammals were not thought to, possess chitin-processing enzymes. Recently, a human chitotriosidase was, described as a marker for Gaucher disease with plasma levels of the enzyme, elevated up to 2 orders of magnitude. The chitotriosidase was shown to be, active against colloidal chitin and is inhibited by the family 18, chitinase inhibitor allosamidin. Here, the crystal structure of the human, chitotriosidase and complexes with a chitooligosaccharide and allosamidin, are described. The structures reveal an elongated active site cleft, compatible with the binding of long chitin polymers, and explain the, inactivation of the enzyme through an inherited genetic deficiency., Comparison with YM1 and HCgp-39 shows how the chitinase has evolved into, these mammalian lectins by the mutation of key residues in the active, site, tuning the substrate binding specificity. The soaking experiments, with allosamidin and chitooligosaccharides give insight into ligand, binding properties and allow the evaluation of differential binding and, design of species-selective chitinase inhibitors.
About this Structure
1LQ0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of human chitotriosidase. Implications for specific inhibitor design and function of mammalian chitinase-like lectins., Fusetti F, von Moeller H, Houston D, Rozeboom HJ, Dijkstra BW, Boot RG, Aerts JM, van Aalten DM, J Biol Chem. 2002 Jul 12;277(28):25537-44. Epub 2002 Apr 17. PMID:11960986
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