1ltb
From Proteopedia
(New page: 200px<br /> <applet load="1ltb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ltb, resolution 2.6Å" /> '''2.6 ANGSTROMS CRYSTA...) |
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caption="1ltb, resolution 2.6Å" /> | caption="1ltb, resolution 2.6Å" /> | ||
'''2.6 ANGSTROMS CRYSTAL STRUCTURE OF PARTIALLY-ACTIVATED E. COLI HEAT-LABILE ENTEROTOXIN (LT)'''<br /> | '''2.6 ANGSTROMS CRYSTAL STRUCTURE OF PARTIALLY-ACTIVATED E. COLI HEAT-LABILE ENTEROTOXIN (LT)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LTB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1LTB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Full crystallographic information is available from [http:// | + | 1LTB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1LTB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: enterotoxin]] | [[Category: enterotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:20:20 2008'' |
Revision as of 14:20, 15 February 2008
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2.6 ANGSTROMS CRYSTAL STRUCTURE OF PARTIALLY-ACTIVATED E. COLI HEAT-LABILE ENTEROTOXIN (LT)
Overview
Biological toxicity of E. coli heat-labile enterotoxin and the closely, related cholera toxin requires that the assembled toxin be activated by, proteolytic cleavage of the A subunit and reduction of a disulfide bond, internal to the A subunit. The structural role served by this reduction, and cleavage is not known, however. We have crystallographically, determined the structure of the E. coli heat-labile enterotoxin AB5, hexamer in which the A subunit has been cleaved by trypsin between, residues 192 and 195. The toxin is thus partially activated, in that it, has been cleaved but the disulfide bond has not been reduced. The, structure of the A subunit in the cleaved toxin is substantially the same, as that previously observed for the uncleaved AB5 structure, suggesting, that although such cleavage is required for biological activity of the, toxin it does not by itself cause a conformational change.
About this Structure
1LTB is a Protein complex structure of sequences from Escherichia coli. The following page contains interesting information on the relation of 1LTB with [Cholera Toxin]. Full crystallographic information is available from OCA.
Reference
Structure of partially-activated E. coli heat-labile enterotoxin (LT) at 2.6 A resolution., Merritt EA, Pronk SE, Sixma TK, Kalk KH, van Zanten BA, Hol WG, FEBS Lett. 1994 Jan 3;337(1):88-92. PMID:8276119
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