This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ly2
From Proteopedia
(New page: 200px<br /> <applet load="1ly2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ly2, resolution 1.80Å" /> '''Crystal structure o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ly2. | + | [[Image:1ly2.jpg|left|200px]]<br /><applet load="1ly2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ly2" size=" | + | |
caption="1ly2, resolution 1.80Å" /> | caption="1ly2, resolution 1.80Å" /> | ||
'''Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)'''<br /> | '''Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1LY2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1LY2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LY2 OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 23: | ||
[[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | [[Category: complement receptor; epstein barr virus; regulator of complement activation; short consensus repeat; viral receptor; complement control protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:21:09 2008'' |
Revision as of 14:21, 15 February 2008
|
Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)
Contents |
Overview
Human complement receptor type 2 (CD21) is the cellular receptor for, Epstein-Barr virus (EBV), a human tumor virus. The N-terminal two short, consensus repeats (SCR1-SCR2) of the receptor interact with the EBV, glycoprotein gp350/220 and also with the natural CD21 ligand C3d. Here we, present the crystal structure of the CD21 SCR1-SCR2 fragment in the, absence of ligand and demonstrate that it is able to bind EBV. Based on a, functional analysis of wild-type and mutant CD21 and molecular modeling, we identify a likely region for EBV attachment and demonstrate that this, region is not involved in the interaction with C3d. A comparison with the, previously determined structure of CD21 SCR1-SCR2 in complex with C3d, shows that, in both cases, CD21 assumes compact V-shaped conformations., However, our analysis reveals a surprising degree of flexibility at the, SCR1-SCR2 interface, suggesting interactions between the two domains are, not specific. We present evidence that the V-shaped conformation is, induced by deglycosylation of the protein, and that physiologic, glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding.
Disease
Known diseases associated with this structure: Systemic lupus erythematosus, susceptibility to, 9 OMIM:[120650]
About this Structure
1LY2 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding., Prota AE, Sage DR, Stehle T, Fingeroth JD, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10641-6. Epub 2002 Jul 16. PMID:12122212
Page seeded by OCA on Fri Feb 15 16:21:09 2008
