1lyd
From Proteopedia
(New page: 200px<br /> <applet load="1lyd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lyd, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...) |
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caption="1lyd, resolution 2.0Å" /> | caption="1lyd, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF T4-LYSOZYME GENERATED FROM SYNTHETIC CODING DNA EXPRESSED IN ESCHERICHIA COLI'''<br /> | '''CRYSTAL STRUCTURE OF T4-LYSOZYME GENERATED FROM SYNTHETIC CODING DNA EXPRESSED IN ESCHERICHIA COLI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. The following page contains interesting information on the relation of 1LYD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb9_1.html Lysozyme]]. Full crystallographic information is available from [http:// | + | 1LYD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. The following page contains interesting information on the relation of 1LYD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb9_1.html Lysozyme]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LYD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase (o-glycosyl)]] | [[Category: hydrolase (o-glycosyl)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:21:14 2008'' |
Revision as of 14:21, 15 February 2008
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CRYSTAL STRUCTURE OF T4-LYSOZYME GENERATED FROM SYNTHETIC CODING DNA EXPRESSED IN ESCHERICHIA COLI
Overview
The polypeptide produced by expressing a chemically synthesized gene, coding for the amino-acid sequence of T4-lysozyme has been crystallized, and subjected to X-ray diffraction. The crystal structure has been refined, to a standard R-factor of 0.191 for data between 8 and 2 A resolution. The, refined model is essentially the same as the well-known structure of, wild-type T4-lysozyme determined previously by Matthews et al. (1987)., Some small changes in the C-terminal region, which is important in, maintaining the folded structure, have been noted. In addition to, confirming that the synthetic gene product is very close to the wild type, this structure provides a benchmark for protein engineering experiments on, the folding and the catalytic activity of this molecule by the method of, gene synthesis.
About this Structure
1LYD is a Single protein structure of sequence from Bacteriophage t4. The following page contains interesting information on the relation of 1LYD with [Lysozyme]. Full crystallographic information is available from OCA.
Reference
Crystal structure of T4-lysozyme generated from synthetic coding DNA expressed in Escherichia coli., Rose DR, Phipps J, Michniewicz J, Birnbaum GI, Ahmed FR, Muir A, Anderson WF, Narang S, Protein Eng. 1988 Oct;2(4):277-82. PMID:3074306
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