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1m5i
From Proteopedia
(New page: 200px<br /> <applet load="1m5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5i, resolution 2.0Å" /> '''Crystal Structure of...) |
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| - | [[Image:1m5i. | + | [[Image:1m5i.jpg|left|200px]]<br /><applet load="1m5i" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1m5i" size=" | + | |
caption="1m5i, resolution 2.0Å" /> | caption="1m5i, resolution 2.0Å" /> | ||
'''Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC'''<br /> | '''Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC'''<br /> | ||
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==Disease== | ==Disease== | ||
| - | Known diseases associated with this structure: Adenoma, periampullary OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id= | + | Known diseases associated with this structure: Adenoma, periampullary OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Adenomatous polyposis coli OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Brain tumor-polyposis syndrome 2 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Colorectal cancer, somatic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Desmoid disease, hereditary OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Gardner syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Gastric cancer, somatic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]], Hepatoblastoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611731 611731]] |
==About this Structure== | ==About this Structure== | ||
| - | 1M5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1M5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:22:05 2008'' |
Revision as of 14:22, 15 February 2008
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Crystal Structure of the coiled coil region 129-250 of the tumor suppressor gene product APC
Contents |
Overview
The APC (adenomatous polyposis coli) tumor suppressor protein has many, different intracellular functions including a nuclear export activity., Only little is known about the molecular architecture of the 2843-amino, acid APC protein. Guided by secondary structure predictions we identified, a fragment close to the N-terminal end, termed APC-(129-250), as a soluble, and protease-resistant domain. We solved the crystal structure of, APC-(129-250), which is monomeric and consists of three alpha-helices, forming two separate antiparallel coiled coils. APC-(129-250) includes the, nuclear export signal NES-(165-174) at the C-terminal end of the first, helix. Surprisingly, the conserved hydrophobic amino acids of, NES-(165-174) are buried in one of the coiled coils and are thus not, accessible for interaction with other proteins. We demonstrate the direct, interaction of APC-(129-250) with the nuclear export factor chromosome, maintenance region 1 (Crm-1). This interaction is enhanced by the small, GTPase Ran in its activated GTP-bound form and also by a double mutation, in APC-(129-250), which deletes two amino acids forming two of the major, interhelical interactions within the coiled coil. These observations hint, to a regulatory mechanism of the APC nuclear export activity by NES, masking.
Disease
Known diseases associated with this structure: Adenoma, periampullary OMIM:[611731], Adenomatous polyposis coli OMIM:[611731], Brain tumor-polyposis syndrome 2 OMIM:[611731], Colorectal cancer, somatic OMIM:[611731], Desmoid disease, hereditary OMIM:[611731], Gardner syndrome OMIM:[611731], Gastric cancer, somatic OMIM:[611731], Hepatoblastoma OMIM:[611731]
About this Structure
1M5I is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The coiled coil region (amino acids 129-250) of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance region 1 (Crm-1)., Tickenbrock L, Cramer J, Vetter IR, Muller O, J Biol Chem. 2002 Aug 30;277(35):32332-8. Epub 2002 Jun 17. PMID:12070164
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