1mq3
From Proteopedia
(New page: 200px<br /> <applet load="1mq3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mq3, resolution 2.8Å" /> '''Human DNA Polymerase...) |
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| - | <applet load="1mq3" size=" | + | |
caption="1mq3, resolution 2.8Å" /> | caption="1mq3, resolution 2.8Å" /> | ||
'''Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine Template Paired with dCTP'''<br /> | '''Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine Template Paired with dCTP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, NA and DCP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1MQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=DCP:'>DCP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:25:13 2008'' |
Revision as of 14:25, 15 February 2008
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Human DNA Polymerase Beta Complexed With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine Template Paired with dCTP
Overview
Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine, (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair, with cytosine or adenine. The ability to accurately replicate through this, lesion depends on the DNA polymerase. We report the first structure of a, polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its, active site. The modified guanine residue is in an anti conformation and, forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate, the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide, flips 180 degrees. Thus, the flexibility of the template sugar-phosphate, backbone near the polymerase active site is one parameter that influences, the anti-syn equilibrium of 8-oxodG. Our results provide insights into the, mechanisms employed by polymerases to select the complementary dNTP.
About this Structure
1MQ3 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Structure of DNA polymerase beta with the mutagenic DNA lesion 8-oxodeoxyguanine reveals structural insights into its coding potential., Krahn JM, Beard WA, Miller H, Grollman AP, Wilson SH, Structure. 2003 Jan;11(1):121-7. PMID:12517346
Page seeded by OCA on Fri Feb 15 16:25:13 2008
Categories: DNA-directed DNA polymerase | Homo sapiens | Single protein | Beard, W.A. | Grollman, A.P. | Krahn, J.M. | Miller, H. | Wilson, S.H. | DCP | MG | NA | Dna | Transferase
